N- or C-terminal fusion polypeptides showed no fluorescence upon transfection of HEK cells. When GFP was inserted into the cytoplasmic loop connecting putative transmembrane regions M3 and M4, the γ/GFP and ε/GFP subunits were fluorescent and formed together with the α, β, and δ subunits ...
To distinguish cells expressing N- or C-terminal GFP fragments by a fluorescent maker of the nucleus, the reporters also encode fusion proteins of histone H2B protein, and red fluorescent protein mCherry or blue fluorescent protein Azurite (Supplementary Fig. 1a). The GRAPHIC system efficiently ...
We fused monomeric enhanced GFP (mEGFP) to the N- or C-terminus of the ubiquitously expressed Drp1 isoform 3 (699 aa) (Fig.1A). N-terminally tagged mEGFP-Drp1 and C-terminally tagged Drp1-mEGFP were overexpressed inE. colifor recombinant protein production and biochemical and biophysical charac...
CD36, an 88 kDa membrane glycoprotein, is found in several cell types and it has been characterized to have two hydrophobic domains at their N- and C-termini which are essential for protein folding and targeting. In this study, we first tagged the green fluorescent protein (GFP) to both ...
该GFP-Tag Polyclonal Antibody. Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localizati
Background:Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein co...
Ca2+ activation of hSlo K+ channel is suppressed by N-terminal GFP tag The human slow poke (hSlo) K + channel was tagged with GFP (green fluorescent protein) at the N-terminus of its 伪-subunit. The fusion protein was express... S Communication,E Meyer,P Fromherz - 《European Journal...
供应商:Agrisera 规格:200 μl 目录价:0 库存状态: CAS编号: 应用范围: 种属来源: 相关信息: 保存条件: 说明书地址:点击查看详细 打印此页关闭此页 上一个:DNA-binding storekeeper protein-related transcriptional regulator[AS11 1774/100 μg] 下一个:N-GFP | N-terminal of GFP[AS11 1776/200 μl]...
sequencing_primer_3EGFP-N sequencing_primer_5na site_3_destroyed site_5_destroyed entrez_gene aliasesHMG20 geneUBC id7316 genbank_ids mutation nameUbiquitin C promoter shRNA_sequence size1211 species 9606 Homo sapiens tags locationC terminal on backbone ...
Protein tags are protein or pepde sequences located either on the C- or Nterminal of the target protein, which facilitates one or several of the following characteriscs: solubility, detecon, purificaon, localizaon and expression. Green fluorescence protein(GFP) is a protein composed of 238 amin...