Synthetic N-Terminal coding sequenceBacillus subtilisN -acetylneuraminic acidN-terminal coding sequences (NCSs) of genes significantly influence gene expression at the translation level and are important for fin
The coding sequence for the N-terminal proPO-peptide was inserted into the pET32a expression vector. Escherichia coli strain Rosetta gami B (Novagen), and the recombinant protein was purified on Ni-NTA beads (GE healthcare). In detail: E. coli cells were grown in LB medium containing 100 ...
We computed ω, the ratio of nonsynonymous (dN) to silent (dS) mutations from a multiple alignment of Src, Yes and Fyn coding sequences of seven primate species, covering 74 million years of evolution. In such framework, neutral selection is associated with an ω-value close to 1, while ...
For biochemical studies, proteins were produced by cloning the coding sequence of KLHL221–178, KLHL121–161, and NRF21–100 into a modified pET28a (+) vector with an N-terminal His6-MBP tag and a TEV protease cleavage site (ENLYFQG). For experiments in intact cells, full-length CUL...
Using DNA microarrays representing approximately 95% of the protein-coding open reading frames in B. subtilis, we compared the expression profiles of wild-type and comK strains, as well as of a mecA mutant (which produces active ComK in all the cells of the population) and a comK mecA ...
We thus created a Naa30A mutant animal where almost all the coding sequence of Naa30A gene was deleted (Supplementary Fig. 12a). This resulted in the deletion of the first 302 amino acids of Naa30A, with an additional frameshift mutation within the remaining coding sequence and a stop codon...
[24], coupled by flexible spacer. This peptide was fused to the N-terminal of the mature coding sequence of VSV-G (αMHC/VSV-G). Although certain anti-MHC-I monoclonal antibodies are known to inhibit HIV production, B9.12.1 appears to have a minor effect on the viral life cycle [...
aAn intron from the castor bean catalase gene CAT-1 was fused within the N-terminal part of the sGFP(S65T) coding sequence to discriminate between Agrobacterium and plant expression (because bacteria cannot splice the intron). 因为细菌不可能接合intron,一intron从蓖麻籽过氧化氢酶基因CAT-1在() ...
which is a metabolite of tryptophan. Multiple alternatively spliced transcript variants that encode different proteins have been described for this gene. This gene shares 5' exon structure with the RNA binding motif protein, X-linked-like 1 locus on chromosome 1, but the coding sequences are non...
We thus generated a series of constructs with different truncations of the N-terminal coding sequence of Adh2 fused to Renilla luciferase, followed by the C-terminal 3×FLAG tag in the original genome locus of Adh2 (Figure 2E). We found that the first 9 amino acids of Adh2 were enough...