PrP(Sc) typing by N-terminal sequencing and mass spectrometry. Arch. Virol 2000:209-216.Chen SG, Zou W, Parchi P, Gambetti P. PrP(Sc) typing by N-terminal sequencing and mass spectrometry. Arch Virol Suppl 2000; 16: 209-16.Chen SG, Zou W, Parchi P, Gambetti P (2000) PrP(Sc) ...
(2004) Snake venomics: characterization of protein families in Sistrurus barbouri venom by cysteine mapping, N-terminal sequencing, and tandem mass spectrometry analysis. Proteomics 4: pp. 327-328Juárez P, Sanz L, Calvete JJ (2004) Snake venomics: characterization of protein families in Sistr...
US5807748 1994年7月8日 1998年9月15日 City Of Hope N-terminal protein sequencing reagents and methods which form amino acid detectable by a variety of techniquesUS5807748 * Jul 8, 1994 Sep 15, 1998 City Of Hope N-terminal protein sequencing reagents and methods which form amino acid ...
N-terminal sequencing The mammalian cell expressed PD-1 protein samples were first applied to SDS-PAGE and then transferred to a polyvinylidene fluoride (PVDF) membrane. The PVDF blot membrane was stained and destained until the protein bands became visible46. The PVDF membrane was dried and the...
ConclusionThedevelopedmethodwassuitablefor theN-terminalaminoacidsequencingof McAbwithpyroglutamateblockage. 结论该方法适于具有焦谷氨酸封闭N-端的单抗的N-端氨基酸序列分析。 www.ceps.com.tw 2. ConclusionsThis result indicatethatPCRprimerintroducedV regionNterminalchangesmayseriouslyaffecttheantigenbindingactivity. ...
The secretory signal sequence was chosen because (i) it directs EXP1 into the secretory pathway in both the parasite and a reconstituted heterologous in vitro system (14) and (ii) N-terminal sequencing of the mature protein had revealed a signal peptidase cleavage site at Ala-22–Glu-23 (...
The N-terminal sequencing characterisation identified 19 proteins, whereas four proteins were found to be blocked and one did not match with any proteins in the database. Most of the water-soluble proteins belonged to a family of alpha -amylase inhibitors. One protein, assigned to chromosome 4...
Dysregulated epigenetic states are a hallmark of cancer and often arise from genetic alterations in epigenetic regulators. This includes missense mutations in histones, which, together with associated DNA, form nucleosome core particles. However, the onc
The performance of the method has been tested with several synthetic peptides and applied to an efficient sequencing of the peptide map, using a nano-scale LC coupled on-line to a tandem mass spectrometer. 展开 关键词: Derivatization Fragmentation MS Peptide sequencing Protein identification ...
Using whole-exome sequencing of germline DNA samples, we identified a heterozygous single-nucleotide change in GP1BA (exone2:c.176T>G), encoding a p.Leu59Arg substitution in the N-terminal domain, segregating with macrothrombocytopenia. This variant has not been previously reported. We also ...