N端编码序列(N-terminal coding sequence,NCS)是一种重要的基因表达调控元件,通过影响核糖体在翻译起始阶段的效率来调控基因表达。NCS编码序列中氨基酸残基的丰度与基因表达水平密切相关,一些氨基酸残基如天冬酰胺(N)、赖氨酸(K)、谷氨酸(E)和蛋氨酸(M)的丰度与基因表达水平呈正相关,而丝氨酸(S)、色氨酸(W)、甘氨
N-terminal coding sequences (NCSs) of genes significantly influence gene expression at the translation level and are important for fine-tuning gene expression in bacteria, however, engineering NCSs to fine-tune metabolic pathways is challenging. Here, we developed a statistics-guided native and ...
蛋白质N-端编码序列(NCS)由于邻近翻译起始区,其同义突变对表达量的影响最为显著。 江南大学刘松研究团队在ACS Synthetic Biology上发表题为Rational Design of the N-Terminal Coding Sequence for Regulating Enzyme Expression inBacillus subtilis的文章,建立了一个统计模型来预测NCS对枯草芽孢杆菌蛋白表达的影响,并基于...
该研究表明NCS可以作为合成生物学工具箱用于微调代谢工程的基因表达。 相关研究发表于《Metabolic Engineering》:Synthetic N-terminal coding sequences for fine-tuning gene expression and metabolic engineering in Bacillus subtilis。 (徐佳琪 摘译) 发布于 2023-12-29 11:00・福建 江南大学 基因检测 基因 ...
4. Tian R, Liu Y, Chen J, Li J, Liu L, Du G, Chen J., Synthetic N-terminal coding sequences for fine-tuning gene expression and metabolic engineering in Bacillus subtilis. Metabolic Engineering. 55, 131-141 (...
EGCG (458.4 Da, purity ≥95%) from green tea was ordered from Sigma-Aldrich (St. Louis, MO). Full-length p53, p53 NTD, full-length MDM2 and MDM2 p53 binding domain were overexpressed and purified59. Briefly, p53 NTD coding sequences were inserted downstream of theThermoanaerobactor te...
Due to the sequence identity between nesprin-2 variants, our knockdown strategy potentially targeted both KASH-less N-terminal and the KASH-containing nesprin-2 giant variants [5]. Importantly, we show that U2OS cells lack nesprin-2 giant and β-catenin transcriptional activity was enhanced by ...
Naa15 and Naa10 coding sequences were PCR-amplified usingC. thermophilumcDNA as template and a set of primers (Supplementary Table 4). The PCR product was ligated into the TOPO Vector (TOPO TA Cloning Kit, Invitrogen). The NcoI restriction sites present in the coding sequences were mutated...
N-terminal flanking sequence affected the cleavage of CLV3 To examine if residues flanking the N-terminus of the CLV3 peptide are required for internal cleavage, we chemically synthesized the CLV3p12 and peptides with additional 1, 3, 4 or 5 residues, namely L-CLV3p13, EEL-CLV3p15, HEEL...
As shown in Figure 1, the ribosomes are targeted to this location by the N-terminal signal sequence of the nascent protein: here they associate with the signal recognition particle (SRP), an association which leads to translation being temporarily halted. The SRP dissociates from the complex, ...