2025年1月7日,中国农业科学院农业资源与农业区划研究所易可可研究员和徐磊副研究员在Plant Communications发表了题为 “The basal level of salicylic acid represses the PRT6 N-degron pathway to modulate root growth and stress response in rice” 的研究论文。该研究揭示了本底水杨酸通过抑制PRT6 N-degron通路...
有意思的是,细胞内以Ser和Ala开端的蛋白存在高度的N端乙酰化修饰(N-terminal acetylation),分别约为99%和95%。所以正常生理条件下,N端乙酰化免除了Non-ac/N-degron pathway对蛋白的降解,同时反过来看,Non-ac/N-degron通路会降解未被乙酰化蛋白...
Protein amino (N) termini are major determinants of protein stability in the cytosol of eukaryotes and prokaryotes, conceptualized in the N-end rule pathway, lately referred to as N-degron pathways. Here we argue for the existence of N-degron pathways in plastids of apicomplexa, algae, and ...
The N-degron pathway targets proteins that bear a destabilizing residue at the N terminus for proteasome-dependent degradation1. In yeast, Ubr1—a single-subunit E3 ligase—is responsible for the Arg/N-degron pathway2. How Ubr1 mediates the initiation of ubiquitination and the elongation of the...
The N-degron pathway, formerly the N-end rule pathway, is a protein degradation process that determines the half-life of proteins based on their N-terminal residues. In contrast to the well-established in vivo studies over decades, in vitro studies of this pathway, including biochemical characte...
To validate our results, we determined the structures of select proteins in the N-degron pathway and compared them with the Protein Data Bank–deposited proteins. Furthermore, several biochemical applications of this technique were introduced. Therefore, this technique can be used as a general tool...
近期,基础医学院宓文义教授和董城教授两个课题组合作于Nature Communications上发表题为CRL2ZER1/ZYG11B recognizes small N-terminal residues for degradation的文章,报道了一种新型的N端降解通路Non-ac/N-degron pathway。在这个通路中,E3连接酶ZE...
the N-degron pathwayAberrantly elevated steroid receptor coactivator-1 (SRC-1) expression and activity are strongly correlated with cancer progression and metastasis. Here we report, for the first time, the development of a proteolysis targeting chimera (PROTAC) that is composed of a selective SRC...
the N-degron pathwayAberrantly elevated steroid receptor co゛ctivator(SRC expression and activity are strongly correlated with cancer progression and metastasis. Here we report, for the first time, the development of a proteolysis targeting chimera (PROTAC) that is composed of a selective SRCbinder ...
Here, we show that the N-degron pathway mediates pexophagy, in which damaged peroxisomal fragments are degraded by autophagy under normal and oxidative stress conditions. This degradative process initiates when the Nt-Cys of ACAD10 (acyl-CoA dehydrogenase family, member 10), a receptor in pexo...