It differs from the related bacterial N-acylglucosamine 6-phosphate 2-epimerase in two respects: (1) the animal enzyme utilizes the free N-acylhexosamines, not the corresponding 6-phosphate esters; and (2) the animal enzyme requires adenosine triphosphate (ATP) as a catalyst. The role of ...
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This chapter discusses the determination of N -acylglucosamine 6-phosphate 2-epimerase. This enzyme is detected in a number of bacteria; the level of activity is greatly increased when the cells are grown in a mineral medium containing N -acetylmannosamine or N -acetylglucosamine. Two assay ...
In serogroup C Neisseria meningitidis, the cssA ( siaA ) gene codes for an UDP- N -acetylglucosamine 2-epimerase that catalyzes the conversion of UDP- N -a... R Colicchio,C Pagliuca,S Ricci,... - 《Infection & Immunity》 被引量: 0发表: 2019年 The tetrameric structure of sialic acid...
29kgofNeu5Acwasobtainedfrom27kgofGlcNAc.Theproductwasrecoveredbydirectcrystallization,andveri®edasidenticaltoauthenticNeu5Ac.#1998ElsevierScienceLtd.AllrightsreservedKeywords:N-acetyl-d-glucosamine;N-acetyl-d-mannosamine;N-acetylneuraminatelyase;N-acyl-d-glucosa-mine2-epimerase;SialicacidN-Acetylneurami...
Complete enzymatic synthesis of sialic acid with N-acylglucosamine 2-epimerase. Journal of Bioscience and Bioengineering . 2002Maru I, Ohnishi J, Ohta Y, Tsukada Y: Why is sialic acid attracting interest now? Complete enzymatic synthesis of sialic acid with N-acylglucosamine 2-epimerase. J Bio...
Complete enzymatic synthesis of sialic acid with N-acylglucosamine 2-epimerase. Maru,I,Ohnishi,J,Ohta,Y,Tsukada,Y. Journal of Bioscience and Bioengineering . 2002Maru I.Why is sialic acid attracting interest now? Complete enzymaticsynthesis of sialic acid with N-acyglucosamine2-epimerase[J].J ...
A method for preparing N-acyl-D-glucosamine 2-apimerase comprises: a step of preparing a recombinant expression vector, pET24a/Nacyl-D-glucosamine 2-epimerase; a step of culturing a microorganism transformed by the recombinant vector to induce expression of N-acyl-D-glucosamine 2-epimerase; and...
Complete enzymatic synthesis of sialic acid with N-acylglucosamine 2-epimerase[J].Biotechnology and Bioengineering,2002.258-265.Maru,I,Ohnishi,J,Ohta,Y,Tsukada,Y.Why is sialic acid attracting interest now? Complete enzymatic synthesis of sialic acid with N-acylglucosamine 2-epimerase. Journal of ...
N-Acyl-d-glucosamine 2-epimeraseEpimerizationMechanistic modelSialic acidSite-directed mutagenesisEnzymatic cascade reactions, i.e. the combination of several enzyme reactions in one pot without isolation of intermediates, have great potential for the establishment of sustainable chemical processes. However,...