Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional描述基因信息说明书与实验方案研究信息 MyoD抗体(G-1)是一种IgG2b κ小鼠单克隆MyoD抗体(也称为MYOD1抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的MyoD蛋白。MyoD抗体(G-1)既可以是非偶联的...
The following antibodies and working dilutions were used for immunofluorescence. MyoD:monoclonal antibody(mAb) 5.8A (1:50 dilution; catalog # 554130, BD Pharmingen); Myf-5: rabbit polyclonal antiserum (1:200 dilution; catalog # sc-302, Santa Cruz Biotechnology);Myogenin: mAb F5D (1:1 dilutio...
sigma aldrich(西格玛) | Invitrogen(英骏) | Millipore | Abcam | CST | Roche | peprotech | prospec | pierce | BD Pharmingen | BD Biocoat | R&D | BioVision | Vector | Enzo life | eBioscience | ABR | Amresco | Cayman Chemical | 脂蛋白 | Merck | Qiagen(凯杰) | Santa cruz(三塔抗体...
Afterwards, 150 mM KCl and 0.2% TritonX-100 were added and immunoprecipitation was performed with a-Flag-coupled magnetic beads (M2; Sigma) or an a-MyoD specific antibody (5.8A, Santa Cruz Biotechnology) for at least 16 h at 4 °C under rotation. Then samples were washed twice with ...
To examine the first possibility, we stain NPCs with the Nup153 antibody and the cell nucleus (by using the H3k4me3 antibody) and imaged them using confocal microscopy. A representative image of one MSC grown on the Control is reported in Fig. 8a. Already qualitatively Fig. 8a suggest that ...
200 dilution), anti-MRF4 (sc-301, Santa Cruz Biotechnology, 1:200 dilution), anti-desmin (AB907, Chemicon, 1:500 dilution), anti-p53 antibody (sc-99 and Pab 240, Santa Cruz Biotechnology, 1:100 dilution), and a 1:2,500 dilution of anti-α-tubulin antibody (Sigma, 1:2,500 ...
Efficiency of lentivirus infection was confirmed with Western blot using MYOD antibody (Ab) as well as eGFP transduction rate. The transduction rate was approximately 80% of infected hAFS cells. BALB/cSlc-nu mice were anesthetized with intramuscular injection of 80 μl of a solution (33.8% ...
an Antibody by Santa Cruz Biotechnology Applications: WB Reactivity: Mus musculus (House mouse) (sc-7150) PARP-1 Antibody (H-250) an Antibody by Santa Cruz Biotechnology (Product has been discontinued) Applications: WB Reactivity: Mus musculus (House mouse) (sc-8298) p57 Antib...
The following dilutions were used for each antibody: Myogenin (Santa Cruz Biotechnology; 1:2 000), YY1 (Santa Cruz Biotechnology; 1:2 000), Troponin (Sigma; 1:2 000), MyHC (Sigma; 1:2 000), α-Actin (Sigma; 1:2 000), α-Tubulin (Sigma; 1:5 000), MyoD (Santa Cruz ...
(1 × PBS and 5 mg/ml BSA-fraction V) at R.T. for 3 h or at 4 °C overnight. The beads were then added to the chromatin/antibody and incubated for 4 h at 4 °C with rotation. Next, the beads were washed twice with cold low-salt wash buffer (20 mM Tris pH ...