used this antibody 1:100 the same day it arrived for western blot. it did not work. With the same membrane I blotted for phospho-MLC2 (Rabbit antibody0, and it works beautifully. 使用Google 翻译 是否有用? 来自 圣克鲁斯生物技术 的回答: ...
Primary antibodies were: anti-MRTF-A (clone C-19; Santa Cruz Biotechnology), anti-phalloidin–tetramethylrhodamine isothiocyanate (TRITC) (P-1951; Sigma-Aldrich). The secondary antibody was anti-goat TRITC (Jackson ImmunoResearch). Electron microscopy Samples were washed in 1× PBS, then fixed in...