Analysis of acute myeloid leukemia cells by flow cytometry, introducing a new light- scattering classification. J Cancer Res Clin Oncol 1994;120:553-557.Harada N, Okamura S, Kubota A, Shimoda K, Ikematsu W,Kondo S, Harada M, Niho Y. Analysis of acute myeloid leukemia cells by flow ...
Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of cells generated during a large array of pathologic conditions ranging from cancer to obesity. These cells represent a pathologic state of activation of monocytes and relatively immature neutrophils. MDSCs are characterized by a ...
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up. J. Vis. Exp. (133), e56976, doi:10.3791/56976 (2018). Download Citation Reprints and Permissions JoVE Privacy ...
Cells treated with Tat and gp120 showed a signifiacntly increased expression of TREM-1 protein compared to control cells treated with vehicle (Fig. 2b). Expression of TREM-1 protein was determined at 24 hours in cells treated with Tat or gp120 using flow cytometry, as shown in Fig....
multiparameter flow cytometryqualitativequantitativereal-timeRT-PCRDetection and quantification of minimal residual disease (MRD) cells in acute myeloid leukemia ... GJ Schuurhuis - 《Lancet》 被引量: 53发表: 2014年 Early assessment of minimal residual disease in AML by flow cytometry during aplasia...
A flow cytometry method has been introduced into the routine investigation of whole bone marrow samples following red blood cell lysis on the basis of a primary CD45/side scatter (SSC) gating procedure. Blast cells were first identified by CD45/SSC gating in 74 cases of acute myeloid leukemia...
Initially, AML is a disease of hematopoietic stem cells (HSC) characterized by arrest of differentiation, subsequent accumulation of leukemia blast cells, and reduced production of functional hematopoietic elements. Heterogeneity extends to the presence of leukemia stem cells (LSC), with this dynamic ...
Flow cytometry data were expressed as percentage of positive cells. Analysis of erythroid cells showed higher proportions of immature cells (P<0.001) and decreased levels of CD71 expression on nucleated red cells (P=0.02) in MDS. Analysis of myeloid cells showed lower proportions of CD10+and ...
Cells were pulsed with CellTrace Violet and activated with Mouse T-Activator CD3/CD28 beads at a 1:1 ratio in the presence of 10 ng/mL of IL-7 for 3 days. Cell proliferation was analyzed by flow cytometry gating on CD4+ or CD8+ T cells. In parallel, we discovered that there is a...
(MHC) class II molecule, HLA‐DR, an important marker of mature lymphoid and myeloid cells. Today, the phenotypic characterizations of MDSCs, consisting of M-MDSCs and G-MDSCs, are relatively well defined in humans by means of flow cytometry (Table 1). In addition, another population of ...