Flow Cytometric Analysis of Myeloid Cells in Human Blood, Bronchoalveolar Lavage, and Lung Tissues Clear identification of specific cell populations by flow cytometry is important to understand functional roles. A well-defined flow cytometry panel for my... YRA Yu,DF Hotten,Y Malakhau,... - 《...
5c). Next, we used flow cytometry to measure cell-type-specific biodistribution in target organs. 3,3′-Dioctadecyloxacarbocyanine perchlorate (DiO)-labelled discoidal IL4-aNPs accumulate in myeloid cells, most notably monocytes and neutrophils, in both spleen and bone marrow, while they do not ...
A flow cytometry method has been introduced into the routine investigation of whole bone marrow samples following red blood cell lysis on the basis of a primary CD45/side scatter (SSC) gating procedure. Blast cells were first identified by CD45/SSC gating in 74 cases of acute myeloid leukemia...
T cells from G-PBSC were sorted via flow cytometry. Purified CD3+T lymphocytes were co-cultured with isolated HLA-DR−/lowCD33+CD16−at a HLA-DR−/lowCD33+CD16−to CD3+T cell ratio of 1:1 in the presence of anti-CD3/CD28 beads (Thermo) in RPMI 1640 (Biological Industries) s...
However, the occurrence of a normal physiologic reactive immature T-cell population in treated bone marrow is unknown. To investigate this, we examined 14 post chemotherapeutic bone marrow specimens with a T-ALL MRD flow cytometry panel. This included 9 acute myeloid leukemia (AML) and 5 T-ALL...
Cells were pulsed with CellTrace Violet and activated with Mouse T-Activator CD3/CD28 beads at a 1:1 ratio in the presence of 10 ng/mL of IL-7 for 3 days. Cell proliferation was analyzed by flow cytometry gating on CD4+ or CD8+ T cells. In parallel, we discovered that there is a...
Thus, the combination of CCE and flow cytometric cell sorting resulted in approximately an 800-fold enrichment for CD34+DR– cells. Multiparameter flow cytometric analysis showed that the CD34+DR- populations were contaminated with less than 1% CD3+, CD19+, or CD57+ cells, but contained ...
Flow cytometry is widely used for exploring cell heterogeneity in leukemia; however, it is limited to the choice of surface markers [8]. Bulk population sequencing can probe into the cell genome and transcriptome, but misses the information of individual cells. Moreover, integrative analyses of sa...
One family member, myeloid cell leukemia-1 (Mcl-1), is commonly amplified in TNBC and correlates with a poor clinical prognosis. Here we show the effect of silencing Mcl-1 and Bcl-2-like protein 1 isoform 1 (Bcl-xL) expression on viability in a panel of seventeen TNBC cell lines. ...
Flow cytometry Cell populations in BM-MNCs were identified with the FACSAria III (Becton Dickinson, Franklin Lakes, NJ, USA). 10 × 106cells were washed in PBA (0.5% BSA in PBS) after thawing, blocked with Fc-block (Miltenyi Biotec, Bergisch Gladbach, Germany), stained for 45 min...