Circulating tumor cells (CTCs) are of great interest in cancer research, but methods for their enumeration remain far from optimal. We developed a new small animal research tool called “Diffusein vivoFlow Cytometry” (DiFC) for detecting extremely rare fluorescently-labeled circulating cells directly...
The labeled cells were subsequently analyzed by an LSRII flow cytometer using FCS Express v5 software. Synaptosomes were prepared from the same AD patients by homogenizing brain tissue in 0.32 M sucrose with a cocktail of enzyme inhibitors. This preparation, known as P2, then underwent probing ...
This assay revealed that GFP-labeled fibroblasts were observed up to one- or two- cell layers away from the cancer cells (Fig. 2b), and a small number of GFP-positive fibroblasts were detected by flow cytometry (Supplementary Fig. 7). We also observed that reconstituted-GFP signals were ...
Almost none of the B cells in the peritoneum, a large fraction of which belongs to the Ly1 B subset, were labeled with BrdU over a period of 7 days in 8-month-old animals. 展开 关键词: B cell development B cell subsets BrdU-labeling flow cytometry lymphocyte proliferation in vivo ...
labeled cells. Preliminary experiments indicated 20 hours was the minimum time required to reliably quantify CFSE-cells in lacrimal-gland infiltrates by flow-cytometry (data not shown). Twenty hours after intravenous injection of CFSE-labeled cells, each pair of lacrimal glands was minced with micro...
11 final cell fixation re-suspend the cells in 200 μL of 1% paraformaldehyde-PBS (this step is important to minimize the off-rate of Abs, and to stabilize the Zenon complex) 12 data acquisition acquire the results on FC instrument For non-Zenon custom-labeled Abs, use of the IgG1 ...
D-F. Flow cytometry analysis revealed the percentage of ALDH positive cells in DU145 cells and DU145-PCSCs. G. Exosomes were obtained from the culture medium derived from DU145-PCSCs (scale bars = 100 nm). H. CM-Dil tracer experiments showed the distribution of exosomes in DU145 ...
their fluorescence will be detected in any channel off one of these lasers that has a filter which passes the nanocrystal emission wavelength range. The result of this cross-laser spectral overlap can be seen in Figure 9B. Cells ...
To maximize the fraction of activated OT-I cells, we labeled cells with the cytosolic dye CFSE, transferred only 1 Â 106 CFSE-labeled cells and determined activation by ex vivo flow cytometry of expression of the early CD8+ T cell activation marker CD69 (Fig. 8a). At 12 h after ...
Fresh BM cells (1 × 106) from WT or Ahr–/– mice were transplanted (intravenously) into each irradiated recipient. After 6 weeks, these recipient mice were injected with MC38 or E0771 cells. All donor-derived tdTomato– RBCs or PLTs were further counted by flow cytometry at the ...