BG-flow, a new flow cytometry tool for G-quadruplex quantification in fixed cellsSecondary DNA structuresG-quadruplexFlow cytometryBackground Nucleic acids can fold into non-canonical secondary structures named G-quadruplexes (G4s), which consist of guanine-rich sequences stacked into guanine tetrads...
Cells were not fixed. Lymphocytes were gated. Applications We have summarized below the main applications of Flow Cytometry Immunophenotyping This has become the most common use of flow cytometry, allowing for the simultaneous immune profiling of blood, bone marrow, and any tissue dissociated into ...
Resuspend cells in sheath fluid for immediate analysis, or fix in 0.5 ml of 0.1% paraformaldehyde and store at 2–8°C in the dark. Fixed cells should be analyzed within 18 hours. TOP References Knapp, W., O. Majdic, and...
Cellular DNA content can be measured by flow cytometry with the aim of聽: (1) revealing cell distribution within the major phases of the cell cycle, (2) estimating frequency of apoptotic cells with fractional DNA content, and/or (3) disclosing DNA ploidy of the measured cell population. In...
After centrifugation, the cell pellet was washed 3 X with PBS and centrifuged for 5 minutes.GBM cells were then incubated overnight in the dark in 1 ml PBS containing RNAse (12.5g /ml; Sigma) and propidium iodide (3g/ml; Sigma) and analysed by flow cytometry. For quantification of CD...
Cellular DNA content can be measured by flow cytometry with the aim of聽: (1) revealing cell distribution within the major phases of the cell cycle, (2) estimating frequency of apoptotic cells with fractional DNA content, and/or (3) disclosing DNA ploidy of the measured cell population. In...
Viability kit for 488 and 561 nm excitation was used to differentially stain a mixture of live (left peak) and heat-treated Jurkat cells (right peak). Cells in(A)were not fixed; cells in(B)were fixed in 3.7% formaldehyde following staining. Samples were analyzed by flow cytometry using 48...
Propidium iodide (PI) was used to differentially stain a mixture of live (left peak) and heat-treated Jurkat cells (right peak). Cells in (A) were not fixed; cells in (B) were fixed in 3.7% formaldehyde following staining...
Flow cytometry is combined with highly specific fluorophore-conjugated antibodies that will only bind to the activated forms of molecules. The advances in flow cytometry enable to perform quantitative multiplexed analysis of single cells within heterogeneous populations stained with specific antibodies for ph...
Flow Cytometry Flow cytometry is a system for sensing individual cells in a physiologic saline solution as they move in a focused liquid stream through a fixed laser beam scattering light and emitting fluorescence that is measured and converted into digitized data. ...