Sevoflurane treatment enhanced the phosphorylation of NF-kappa B, and up-regulated the expressions of TLR4 and MyD88 (P < 0.05), which demonstrated that sevoflurane inhibited proliferation and differentiation of neuronal cells by activating TLR4/MyD88/NF-kappa B signaling both in vitro and vivo. ...
88 (MyD88), subsequently delaying the translocation of nuclear factor-kappa B (NF-κB) to the nucleus. Additionally, co-immunoprecipitation (Co-IP) experiments demonstrated that EPS-LM inhibited the binding of TLR4 to MyD88. The ability of EPS-LM to inhibit the TLR4/MyD88/NF-κB pathway, ...
TLR4 (A5258, ABclonal, Woburn, MA, USA), myeloid differentiation factor 88, MyD88 (23230-1-AP, Proteintech, Wuhan, China), nuclear factor kappa B p65, NF-κB p65 (10745-1-AP, Proteintech, Wuhan, China), Phospho-NF-κB p65, P-NF-κB p65 (82335-1-RR, Proteintech, Wuhan, China)...