Gram stainGram stainKlebsiella: Usually cannot penetrate the waxycell wallCell wallThe outermost layer of a cell in most plants; bacteria; fungi; and algae. The cell wall is usually a rigid structure that lies external to the cell membrane, and provides a protective barrier against physical or...
The cerebrospinal fluid (CSF) contained 870 white cells/渭l, most of which were neutrophils; the glucose level in the CSF was 10mg/dl, and the protein level was 140mg/dl. Stained smears of the CSF, including Gram staining and India-ink preparations, disclosed no microorganisms. Capsular ...
摘要: Although the Gram stain is a frequently used technique, its application to mycobacterial identification is confusing. A recent clinical experience is presented to demonstrate the gram-neutrality of the air-dried bacillus.关键词: Humans Mycobacterium tuberculosis Tuberculosis, Pulmonary Staining and ...
They are impermeable to basic dyes such as those used in Gram staining but have been detected by the use of the Ziehl–Neelsen staining procedure. With this staining method, organisms can be distinguished by their red color in between the surrounding blue tissues. 7.1.2 The Global Effect of ...
Although mycobacteria yield slightly positive reactions in the Gram stain, the mycobacterial cell envelope (Figure 1.3) possesses more Gram-negative than -positive features, i.e. a less extensive PG matrix and an outer lipid bilayer which has been identified as like an outer membrane, the ...
(b) In the slough (){the ulcer base there are numerous acid-alcohol fast bacilli (AAFB) (Ziehl-Neelsen stain). lesion consisted of a large diffuse sub-epithelial infiltrate below which a number of more focal opacities were pres ent. A satellite lesion was identified supranasally (Fig....
In all three cases for which Gram stain results were available, blood cultures showed GPRs. An intravascular device was present in 4 of the 5 cases, and the device was removed in 3 cases. Blood cultures were positive within 2 to 5 days of incu- bation for all cases. The prognosis ...
Mycobacterium Avium Complex (MAC) is an established microbiologic cause of pulmonary disease, lymphadenitis, and disseminated disease in cases of advanced immune suppression. However, MAC manifesting as vertebral osteomyelitis is less common, and is part
These organisms can be visualized using acid-fast stains such as the Ziehl–Neelsen, Fite, and Kinyon stains, which fail to decolorize with weak acids in mycobacteria due to the organism's waxlike cell wall. If a Gram stain is performed, bacilli may be weakly Gram-positive or -negative. ...
Acid-fast staining of duodenum revealed numerous intracellular, slender, rod-shaped, acid-fast–positive bacteria in macrophage cytoplasm (arrows) in case of presumed mycobacteriosis. Ziehl–Neelsen stain; bar, 50 μm.Reprinted with permission from Asfaw and Sun (2010). Sign in to download full...