smegmatis and produces large, clear, easily identifiable plaques. The system has been evaluated with clinical specimens in several studies and has been used to discern rifampicin-resistant and rifampicin-sensitive hosts (Albert et al., 2001, 2002a,b, 2004; McNerney et al., 2000; Pai et al....
Transfer of the IS6110 insertion sequence between Mycobacterium smegmatis and the M. tuberculosis complex species, and its discovery in another environmental Mycobacterium sp. strain JLS, suggested that these species once co-occupied a single environmental niche [7]. Reductive evolution of specialized ...
Keywords: Mycobacterium wolinskyi, Rapidly growing mycobacteria, Umbilical cord blood transplant, Unidentifiable Gram-positive rods, Case report Background Mycobacterium wolinskyi is a rapidly growing non-tuber- culous mycobacteria (NTM) that belongs to the Myco- bacterium smegmatis group [1]. ...
smegmatis, M. sphagni, M. szulgai, M. triplex, M. tuberculosis, M. tusciae, M. ulcerans, M. vaccae, M. xenopi.). Marker panels were generated for MTBC and NTM (‘phylo groups’) and for individual species within those groups, using the same method as for staphylococci based on ...
To evaluate the functional role of MSMEG-3762/63 in membrane potential (Δψ), cytofluorimetric analysis was performed onM. smegmatis(wt),M. smegmatis(ΔMSMEG_3763) andM. smegmatis(ΔMSMEG_3763pBD04) complemented strain, using the DiOC2fluorescent carbocyanine dye. DiOC2exhibits green fluoresce...
smegmatis cell extract, M. vaccae cell extract all A450 = 0.05. Full size image Effect of sample treatment on 85 B detection The failed detection of 85 B in Mtb cell extract in sandwich ELISA compared to the successful detection in direct ELISA was further analysed. It was hypothesized ...
Products of phage amplification follow- ing infection are observed as lysed areas (plaques), which can be recorded after 24 h on indicator plates prepared with fast-growing Mycobacterium smegmatis [27]. The phage amplification assay was used to obtain a rapid es- timation (within 24–48 h) ...
M. Fortuitum M. Chelonei M. Smegmatis ? Microbiology = MTB: fastidious, slowly growing, acid alcohol fast, aerobic bacterium (AAFB) = Cell wall composed of complex peptidoglycans and long chain lipids These lipids make MTB hydrophobic thus resistant to many stains routinely used in Laboratory...
Mycobacterium smegmatis biofilm (Oosthuizen, 2015). The resistance/tolerance of the biofilm to antibiotics has been attributed to different factors; these include the low metabolic levels of bacteria in biofilms and the drastically reduced rate of cell division (Brown, Allison, & Gilbert, 1988). ...
smegmatis, M. abscessus and M. kansasii. View article Cellular and molecular mechanisms of internalization of mycobacteria by host cells Luiz E Bermudez, Felix J Sangari, in Microbes and Infection, 2001 Mycobacterium avium, M. tuberculosis and M. leprae are intracellular pathogens that infect and ...