myb-dna-bind怎么分类网友 1 最佳答案 回答者:网友 MYB转录因子以保守的DNA结合域为共同特征,是植物最大的转录因子家族之一,广泛参与植物代谢调控。本文根据植物中MYB转录因子的DNA保守结构域,通过RT-PCR和RACE的方法从大豆品种吉林3号的叶片中分离得到了2个MYB基因GmMYBJ6和GmMYBJ7,并对其结构特征和表达特性进行...
In the light of the structure of Myb-DNA complex, these results indicate that different DNA-binding modes may be used by c-Myb to recognize different classes of binding sites. We also screened the sequence database for potential Myb-binding sites, and found sequences of several promoters that...
你用的 参考基因组 和LOC开头的 参考基因组不一样，两套 ID;
This review will focus on v-Myband its normal cellular counterpart c-Mybwith an emphasis on the biological and biochemical functions of their protein products. Both the v-Myb and c-Myb proteins are nuclear, bind directly to DNA, have short half-lives, and can regulate gene expression. As ...
Many of these transcripts encode a disrupted DNA binding domain. Co-expression analyses were preformed to understand the functions of the grapevine MYBs. An enrichment among the most highly co-expressed genes of each MYB was used to predict a function. VviMYB13 was previously not characterized, ...
[2],它们在植物逆境胁迫响 应中发挥着重要作用.MYB 作为植物中最大的转录 因子家族之一,含有 DNA 结合结构域(DNA-binding domain, DBD), 转录调控区(transcription regulation domain, TRD),寡聚化位点(oligomerization site, OS) 和核定位信号(nuclear location signal, NSL)[3].该 类转录因子通过 DBD 与下游...
region of the DNA alpha-helix binds directly to a primary region of the molecular DNA [8], . TheMYBsfamily is categorized into 4 groups based on the proportion of contiguous MYB repetitions, notably 1R-MYB, 2R-MYB, 3R-MYB, and 4R-MYB, which include 1, 2, 3, and 4 MYB repeats,...
The DNA-binding domain of c-Myb consists of three homologous tandem repeats of 52 amino acids. The structure of the third (C-terminal) repeat obtained by NMR analysis has a conformation related to the helix-turn-helix motif. To identify the role of each repeat in the sequence recognition of...
The DNA oligonucleotides were 5′ end-labeled using T4 polynucleotide kinase in the presence of [γ-32P]ATP. After hybridization, the filters were washed with 3 changes (30 min each) of 0.2× SSC, 0.1% SDS at 65 °C. Radioactive signals were detected using phosphoimaging and quantified ...
在与DNA接触的过程中, 2个MYB重复序列紧密结 合于DNA大沟中, 使2个“识别螺旋”协同作用, 结 合到特定的DNA序列上(Jia等2004)。 根据MYB重复序列数目的不同, MYB家族蛋 白可以分为4种类型: 4R-MYB含有4个重复序列; 3R-MYB (R1R2R3-MYB)含有3个连续的重复序 列; R2R3-MYB含有2个重复序列; MYB-...