functional protein, containing two complete MYB DNA-binding domains (R2 and R3 repeats). ScMYB2S2, with the first MYB DNA-binding domain (R2 repeat) missing in the N-terminal amino acid sequence, might lim- ited its function on directly involved in transcriptional regulation. Similarly, two ...
The MYB protein contains a DNA-binding domain (DBD) at the N-terminal, which consists of three tandem repeat domains of approximately 50 amino acids containing tryptophan, named R1, R2 and R3; a conserved C-terminal negative regulatory domain (NRD); and a transactivation domain (TAD) located...
The animal proto-oncogenec-mybwas the first gene identified to encode a MYB protein6. MYB proteins contain up to three 52-amino acid residue repeats, the R1, R2 and R3 MYB domains, which bind DNA in a sequence-specific manner. The c-myb protein contains three of these DNA-binding domain...
This review will focus on v-Myband its normal cellular counterpart c-Mybwith an emphasis on the biological and biochemical functions of their protein products. Both the v-Myb and c-Myb proteins are nuclear, bind directly to DNA, have short half-lives, and can regulate gene expression. As ...
The c-Myb protein contains three distinct functional domains: a DNA binding domain, a transactivation domain, and a negative regulatory domain (20). Although a c-myb-dependent transcriptional transactivation that is independent of the DNA binding domain has been reported (21), all the remaining c...
While they share similar DNA binding domains and bind to the same DNA sequences, the three family members activate partially distinct sets of genes17,18 and their knock-out mouse models elicit distinct phenotypes (see15). While only the viral, truncated form of c-MYB, v-MYB, appears to ...
Because c- and B-Myb have been reported to bind to identical DNA sequences, we wanted to determine which protein was present in the bands observed in EMSA. Northern analysis showed that the Molt-4 cell line expressed high levels of c-myb RNA and that B-myb mRNA was also present. In ...
The AMV v-Myb protein product is p45v-myb. The E26 v-Myb protein product is p135gag-myb-ets. Both proteins are nuclear. C-Myb contains a DNA-binding domain with three repeat regions, R1, R2, R3, transactivation domain, leucine zipper, trans-repression domain and a conserved domain in ...
In the regulation of flavonoid biosynthesis, MYB repressors mainly exist in the DNA binding domain with two repeats of R2R3-MYB and a single repeat of R3-MYB. Figure 4. Phylogenetic relationships of conserved protein motifs in MYB transcription factor repressors related to anthocyanin biosynthesis ...
The family of avian myeloblastosis proto-oncogene (MYB) transcription factors (TFs) is one of the largest and most abundant of its kind in plants [1]. Each N terminus of MYB family proteins has a highly conserved DNA-binding domain (DBD) consisting of 1–4 incomplete tandem repeats (R)...