In all confocal images, the scale bar is 60 μm and the nuclei were visualized using DAPI (blue) Full size image Fig. 7 OXR1 impacts midbrain development by shaping histone arginine methylations. a Representative bright-field images (40x) show the progression of midbrain organoids, derived...
CMR plotted against population size for varying values of scale parameter S. Population size was varied from 10 up to 1,000 and each individual consisted of 1 gene of 1,000 bp in length. Peak 0 and 1 were given a radius of 2 and 5 respectively, while the distance between their peaks...
Fig. 2: HomozygousMlklD139Vneonates exhibit dispersed upper body inflammation. aMacroscopic appearance ofMlklWt/Wt,MlklWt/D139VandMlklD139V/D139Vmice at postnatal day 3.bCoronal section of mouth and neck region of postnatal day 2 litter mates stained with hematoxylin and eosin (H&E). Dilated...
scale down nearly proportionally with the number of parallel jobs, which can be specified with the argument-j. If the parameter is not specified, then DeCiFer will attempt to use all available CPUs; however, when using a computing cluster, we strongly recommend the user to always specifies-...
Prior to large-scale infections, viral stocks were first titered in U373-MAGI cells based on EGFP expression. The day before infection, 31,250 cells/well were plated in 24-well plates. After 24 h, the media was replaced and varying volumes of virus ranging from 15.625 to 500 µL ...
μm. see alsofigure s5. previous large-scale efforts to decipher cancer-specific mutational signatures in bulk tumor genomes (alexandrov et al., 2013b) discovered 21 unique signatures based on the substitution type and the surrounding two bases. we reasoned that our signatures might have been ...
Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis. Genetics 2003, 164, 731–740. [Google Scholar] [CrossRef] Yan, W.; Deng, X.W.; Yang, C.; Tang, X. The Genome-Wide EMS Mutagenesis Bias Correlates With Sequence Context and Chromatin ...
IMPI requires non-compressed FASTQ files of small-scale NGS data of a gene region of interest. The workflow can be executed for one single file as well as for batches of files. Additionally, all sample-specific information has to be provided. This includes the reference sequence and primer de...
(qs≥ 30 in Phred scale); this is in comparison twice what some DNA-based calling algorithms have used [21]. Finally, only positions in which the minor allele was supported by at least 6 reads were considered (n≥ 6). In combination, these parameters define a theoretical distribution...
2 megabase-scale deletions in 2 crypts from the same cow; the loss of an X chromosome in a female mouse crypt; and a 52-Mb segment with copy-neutral loss of heterozygosity in a human crypt (Extended Data Fig.8, Methods). These results suggest that large-scale somatic copy number changes...