Here, we explored the mutation profiles of UV radiation on the genomic scale. UV radiation is a standard mutagen, and its mutability has been studied extensively due to its ubiquity in nature and convenience of handling. In addition, UV is also applied in many fields such as UV disinfection ...
scale down nearly proportionally with the number of parallel jobs, which can be specified with the argument-j. If the parameter is not specified, then DeCiFer will attempt to use all available CPUs; however, when using a computing cluster, we strongly recommend the user to always specifies-...
The median tumour was predicted to harbor one to three subclones across pipelines (lower range Q1: 1, Q3: 1; upper ranger Q1: 3, Q3: 5), and two randomly-selected successfully executed pipelines would differ by 1.1 ± 1.3 (mean ± SD) in their predicted number of subclones...
Inset: the mutant on a linear scale. Next, we examined the possibility of additional infusions; see Figure 4. With two additional infusions, at Days 21 and 28, the mutant was temporarily controlled but rebounded after the infusions stopped. Figure 4. The case with two additional infusions....
Obviously then, these dates, calculated from the mtDNA analysis, must match the presupposed evolutionary time scale since the calculation is based on this presupposition. The circularity of this method is inconsistent with good scientific method and is worthless as far as independent predictive value ...
Scale bar: 20 μm. d Immunoblot analysis of total protein lysates from brains of wild-type (C9+/+) and C9orf72 knock-out (C9−/−) mice. Only a single band around 50 kDa corresponding in size to the murine isoform 1 is detected with mAbs 12E7 and 1C1 in wild-type mice (...
Prior to large-scale infections, viral stocks were first titered in U373-MAGI cells based on EGFP expression. The day before infection, 31,250 cells/well were plated in 24-well plates. After 24 h, the media was replaced and varying volumes of virus ranging from 15.625 to 500 µL ...
Cooperative takeover also shares many characteristics of traditional phase transition phenomena such as a dramatic spatial reorganization on a scale comparable to the size of the system and the presence of large fluctuations in the neighborhood of the critical point (Figure 5). As has been ...
(qs≥ 30 in Phred scale); this is in comparison twice what some DNA-based calling algorithms have used [21]. Finally, only positions in which the minor allele was supported by at least 6 reads were considered (n≥ 6). In combination, these parameters define a theoretical distribution...
2 megabase-scale deletions in 2 crypts from the same cow; the loss of an X chromosome in a female mouse crypt; and a 52-Mb segment with copy-neutral loss of heterozygosity in a human crypt (Extended Data Fig.8, Methods). These results suggest that large-scale somatic copy number changes...