200 μl Wash Buffer, 1 μl RNase Inhibitor. The IP reactions were incubated for 30 min at 4 °C with rotation. After incubation, 25 μl pre-washed beads were added to each IP reaction, followed by an additional 20 min of rotation at 4 °C. At this point the samples...
large effective population sizes in comparison to human and hence the probability for neo-functionalisation in mice is greater than in humans. Theoretical and population genetics data predict that in large populations strong constraints act against slightly deleterious mutations, hindering the subsequent f...
RNaseOUT Recombinant Ribonuclease Inhibitor Invitrogen Cat#10777019 cOmplete Protease Inhibitor Cocktail Roche Cat#11697498001 Recombinant human CLCF1 protein R&D Systems Cat#962-CL-050 Recombinant human RSPO1 protein Abcam Cat#ab134626 Recombinant mouse IGF-2 protein R&D Systems Cat#792-MG-050 EdU (5...
In gliomas, one main consequence of the R132H mutation is the alteration of the epigenetic pat- terns, as 2HG may act as a competitive inhibitor of histone and DNA demethylases requiring alpha-KG as a cofactor [7-9]. Initially, increased neo-angiogenesis due to increased expression of the...
The hybridized primer-RNA complex was extended in a 80-μl volume containing 7.5 mM KCl, 47.5 mM Tris-HCl, pH 8.2, 10 mM MgCl2, 10 mM dithiothreitol, 0.5 mM dNTPs, 50 units of human placenta RNase inhibitor, and 53 units of avian myeloblastosis virus reverse transcriptase, and heated ...
Sorafenib (SO) is a multi-kinase inhibitor that targets upstream signals in the MAPK pathway. Drug resistance and transient survival benefits are the main obstacles associated with SO treatment in Hepatocellular carcinoma (HCC) patients. Mebendazole (MBZ
需要金币:*** 金币(10金币=人民币1元) fth1在b ovis 019株侵染小鼠巨噬细胞中的功能研究-study on the function of ft h1 in murine macrophages infected by b ovis 019 strain.docx 关闭预览 想预览更多内容,点击免费在线预览全文 免费在线预览全文 ...
S3). To minimize the risk of interfering with the transcription and processing of its host Cdc20b gene, the targeting construct was designed to replace a small portion of the miR-449 cluster locus (∼1.7 kb) with a PGK-Neo cassette flanked by FRT sites (supplemental Fig. S3). This ...
However, such anti-apoptotic and cytoprotective effects of phloroglucinol were greatly abolished by the HO-1 inhibitor, suggesting that phloroglucinol could increase the Nrf2-mediated activity of HO-1 to protect C2C12 myoblasts from oxidative stress. Taken together, our results indicate that ...
Additionally, negative controls containing RNA but no RNAse inhibitor and no reverse transcriptase as well as a negative control without RNA, RNAse inhibitor and no reverse transcriptase and one negative control comprising RNAse inhibitor and reverse transcriptase but no RNA were included as controls ...