10 µg of RNase B are denatured with 1X Glycoprotein Denaturing Buffer (0.5% SDS, 40 mM DTT) at 100°C for 10 minutes. After the addition of NP-40 and GlycoBuffer 2, two-fold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37°C. Separation of...
; 1× reverse transcriptase buffer (50 mM Tris–HCl, pH 8.3, 75 mM KCl, 3 mM MgCl2); 8 mM DTT; 20 U RNase inhibitor (Gibco BRL); and 200 U of MMLV-reverse transcriptase (New England Biolabs, MA, U.S.A.) followed by incubation of the tubes at 37 °C for 60 min. The ...
RNase抑制剂(小鼠源)3000U Murine RNase Inhibitor -20℃保存 RNase抑制剂(猪源)500U 非酶DNA清除剂1.5mL DNA Erasol 4℃保存 非酶DNA清除剂-215次 DNA Erasol-2 4℃保存 柱式DNA清除剂50次 Column DNA Erasol 4℃保存 大提柱式DNA清除剂5次 Maxi Column DNA Erasol 溶液A需4℃保存,其余可以室...
3000U RNase抑制剂(小鼠源) Murine RNase Inhibitor -20℃保存小鼠 FDPS 基因全长ORF克隆 2 ug pCMV-SPORT6 SNAP25 pCMV-SPORT6 SNAP25 低温运输,-20℃保存小鼠 FUNDC2 公司简介 上海西格生物科技有限公司(Shanghai Sig Biotechnology Co., Ltd)是一家生物企业,主要从事免疫学、分子生物学和常规生化试剂的销售...
鼠源RNase抑制剂(Murine RNase Inhibitor) 面议 Streptavidin链霉亲和素 面议 过氧化物酶标记山羊抗兔IgG(H+L) 面议 Human IgG 人免疫球蛋白G 面议 产品简介 Human Recombinant Insulin 重组-人胰岛素本品为基因重组技术制备的全长序列的人胰岛素,分子量大约为5800 Da,结构与天然来源的人胰岛素相同,常用作细胞...
鼠源RNase抑制剂(Murine RNase Inhibitor) 面议 Streptavidin链霉亲和素 面议 过氧化物酶标记山羊抗兔IgG(H+L) 面议 Human IgG 人免疫球蛋白G 面议 产品简介 重组蛋白A/G-Recombinant Protein A/G,天然蛋白A(Protein A)是一种发现于金黄|色|葡萄|球菌的细胞壁表面蛋白,天然蛋白G(Protein G)是一种分离自...
The reverse transcriptase gene (or the genetic information contained therein) can be obtained from a number of different sources, e.g., Moloney Murine leukemia virus (M-MLV); human T-cell leukemia virus type I (HTLV-I); bovine leukemia virus (BLV); Rous Sarcoma Virus (RSV); human immunod...
Another example of such distronic expression vector is pCDE vector, a derivative of pCAVDHFR containing the murine encephalomyocarditis virus internal ribosomal entry site (nucleotides 260 through 824; Jang and Wimmer, Genes and Dev. 4:1560, 1990) cloned between the adenovirus tripartite leader and ...
Spleen cells from antibody producing mice are isolated and fused with murine myeloma cells, such as SP-2/0 or the like, by techniques known to the art, see Kohler and Milstein, Nature 256: 495-497 (1975). Hybridoma cells are selected by growth in hypoxanthine, thymidine and aminopterin ...
Exemplary promoters suitable for mammalian cell systems include, for example, the SV40 early promoter, the cytomegalovirus (CMV) promoter, the mouse mammary tumor virus (MMTV) steroid-inducible promoter, and the Moloney murine leukemia virus (MMLV) promoter. Exemplary promoters suitable for bacterial ...