运费:全国包邮 销量:2836酶活 规格: 2KU 10KU 100KU 200KU 1000 KU 80 KU 2KU-EN 10KU-EN 100-KU-EN 200KU-EN 1000 KU-EN 80 KU-EN 数量: - + 库存: 4996 *本订单仅属于要约邀请,尚不能直接达成交易,具体交易事项由双方于线下完成 产品详情FAQ操作手册买家评论...
Murine RNase inhibitor (40U/μl) 鼠源RNase抑制剂 10701ES05 2 KU 216.00 10701ES10 10 KU 916.00 10701ES20 20 KU 1695.00 10701ES60 100 KU 7796.00 Murine RNase Inhibitor (200 U/μL, Glycerol-free) 10703ES05 2 KU 237.00 10703ES10 ...
储存缓冲液:20 mM HEPES-KOH,50 mM KCl,8 mM DTT,50% Glycerol,pH 7.6 @ 25°C。 注意事项: 1.高浓度的尿素,胍盐等蛋白变性剂作用下,Murine RNase Inhibitor会失活。 2.Murine RNase Inhibitor的使用量按照终浓度1U/μl添加。 3.Murine RNase Inhibitor应在可能导致RNase污染的其他成分之前加入反应中。
In brief, cells were lysed in lysis buffer (20 mM Tris pH 7.5, 150 mM NaCl, 5 mM MgCl2, 0.2% NP40, 5% glycerol, 1 mM DTT) supplemented with PhosSTOP phosphatase inhibitor cocktail (Roche) and cOmplete™ EDTA-free protease inhibitor cocktail (Roche). Following centrifugation, ...
Cells were harvested in an IP buffer containing: 20 mM Tris, pH 7.5, 150 mM NaCl, 5 mM EDTA, 5 mM beta-glycerolphosphate, 5 mM NaF, 10% Glycerol and 0.1% BME, 0.5% Triton, and protease and phosphatase inhibitors (1:100) by scraping the cells and then incubating for 1 h at 4 ...
Glucose metabolic pathways are associated with lipid metabolism, notably through de novo synthesis of fatty acids and glycerol [45]. Based on these data we decided to analyze glucose metabolism in vitro and in vivo after LL35 depletion. Total cellular ATP is predominantly generated by glycolysis ...