In conclusion, our study demonstrates that the applied murine iPSC-microglia and iPSC-macrophage culture models are able to recapitulate in vivo microglia and monocyte/macrophage ontogeny and corresponding phenotypical/functional properties upon classical and alternative immune polarisation, and therefore ...
By electron microscopy, immunomagnetic bead-purified CD11b+ cells in UV-EC were comprised of neutrophils, differentiated macrophages, and mononuclear cells with prominent lysosomes, but no Birbeck granules; the class II MHC+ subset resembled a monocytic cell in between differentiated macrophages and ...
The RAW264.7 murine monocyte/macrophage cell line was cultured in Dulbecco's modified Eagle's medium and induced to differentiate into bone resorbing osteoclasts by 10 ng/ml receptor activator of NF-kB ligand (RANKL; 462- TEC-010, R&D Systems, Wiesbaden, Germany) in minimal essential medium ...
An unbalanced monocyte macrophage polarization in the bone marrow microenvironment of patients with poor graft function after allogeneic haematopoietic ste... Poor graft function (PGF) is a severe complication of allogeneic haematopoietic stem cell transplantation (allo-HSCT). Murine studies have demonstrate...
we found significant increases in numbers of cells stained for this marker in the secondary spongiosae after cPTH infusion in WT mice but low staining in MCP-1−/−mice even after cPTH infusion, demonstrating that MCP-1 is necessary for monocyte and macrophage recruitment in bone marrow (...
To enrich the macrophage population, we supplemented complete medium with 25 ng/ml murine GM-CSF (Miltenyi Biotech, Bergisch Gladbach, Germany) for GM-CSF grown bone marrow derived macrophages (GM-BMMs) or 20% L929 murine fibrosarcoma cell line culture supernatants for M-CSF grown bone marrow ...
Furthermore, kinetic analysis revealed that the reduction in intracellular and plasma membrane-bound class II molecules started ≈ 20 h after infection. While MHC class II antigens were most prominently reduced in parasite-positive host cells, culture supernatant from T. gondii -infected BMM ...
B16-F10 melanoma cells were incubated (30 min, 37 °C) with 0.02 mM mannan-BAM in culture medium and subsequently washed. The suspension of bone marrow neutrophils (90% purity), primed with GM-CSF + TNF-alpha + laminarin in culture medium was added to B16-F10 (both free and mannan-...
Aortic dissection is a life-threatening condition, which is characterised by separation of the constituent layers of the aortic wall. We have recently shown that monocyte/macrophage infiltration into the aortic wall is a pathogenic mechanism of the condi
In this present study, we aim for the first time to investigate the functional effect of AF16 on glioblastoma survival in vivo and on the monocyte/macrophage population, with the emphasis on implications for the TME of glioblastoma. We also analyzed CD8+T cells, M0 macrophages/microglia, and ...