Murine bone marrow-derived macrophages constitute feeder cells for human B cell hybridomas. J Immunol Methods 1996; 196:85±91.Hoffmann P, Jimenez-Diaz M, Weckesser J, Bessler WG. Murine bone marrow-derived macrophages constitute feeder cells for human B cell hybridomas. J Immunol Methods ...
Fig. 1:Toxoplasma gondiigenome-wide loss-of-function screen in naïve or IFNγ-activated murine bone-marrow-derived macrophages. aScreening workflow. At least 5 × 108Cas9-expressing RH parasites were transfected with linearized plasmids containing 10 sgRNAs against everyToxoplasmagene. Transfecte...
Interferon (IFN)-γ, a key immunoregulatory cytokine, has been thought to be produced solely by activated T cells and natural killer cells. In this study, we show that murine bone marrow– derived macrophages (BMMΦ) secrete large amounts of IFN-γ upon appropriate stimulation. Although interle...
Slamf9 expression is upregulated by B16F1-derived tumor-conditioned medium in murine bone marrow-derived macrophages By cDNA-microarray analysis we examined the effect of tumor-conditioned medium (TCM) from B16F1 cells on the gene expression profile of bone marrow-derived macrophages (BMDM) in vitr...
Shrivastava, “Involvement of RAS and MAP Kinase (ERK-1) in Cisplatin-Induced Activation of Murine Bone Marrow-Derived Macrophages,” Biochemistry and Molecular Biology International, Vol. 45, 1998, pp. 527-534. [26] J. Dong, S. Ramachandiran, K. Tikoo, Z. Jia, S. S. Lau and T. ...
The bone marrow-derived macrophages were plated in 96-well microculture plates at a density of 105 cells/well in Dulbecco's minimal essential medium supplemented with 2 mmol/L l-glutamine and 2 × 10−5 mol/L β-mercaptoethanol and stimulated with 100 ng/ml lipopolysaccharide (LPS) (...
Proliferation of three murine marrow-derived stromal cell lines, LC1, LC2, and LC3, depended on initial cell density. For LC2 and LC3, the cell density-dependence was negated by conditioned-media, indicating growth dependence on a soluble growth factor. For LC1, conditioned-media failed to stim...
Gamma interferon, tumor necrosis factor alpha, and macrophage colony-stimulating factor 1, or combinations thereof, were shown to endow murine bone marrow-derived macrophages with significant amebicidal activity. Low doses of gamma interferon and tumor necrosis factor alpha and of gamma interferon and...
In addition, the assay has been used to measure extracellular DNA uptake by murine bone marrow-derived macrophages. Thus, the protocol described herein appears to be broadly applicable for use in different species and cells. The use of EdU labeling for the exogenous DNA allows for the use of ...
Murine resident bone marrow macrophages (RBMM), which form intimate associations with developing haematopoietic cells in situ, were isolated as intact clusters by collagenase digestion. After purification, RBMM were found to express high levels of a novel haemagglutinin for unopsonized sheep erythrocytes...