多重PCR( multiplex polymerase chain reaction,MPCR)也称复合PCR,是在常规PCR基础上改进并发展起来的一种新型PCR扩增技术,即可在一个反应体系中加入两对以上引物,同时扩增出多个核酸片段,由 Chambehian'于1988年首次提出,其反应原理、反应试剂和操作过程与常规PCR相同。多重PCR既有单个PCR的特异性和敏感性,又较之快捷...
Multiplex polymerase chain reaction (mPCR)-denaturing high-performance liquid chromatography (DHPLC) primers and method for detecting and identifying mycobacterium本发明提供了一组用于检测分枝杆菌及鉴别致病分枝杆菌的五重mPCR-DHPLC方法中使用的核酸,包括核苷酸序列分别如SEQ ID No.1和SEQ ID No.2,SEQ ID No...
multiplexpolymerasechainreaction(MPCR)ChenMingjieLect.;CollegeofLife ci.&Tech.,HuazhongUniv.of ci.&Tech.,Wuhan430074,China.聚合酶链式反应(PCR)技术是转基因植物分子鉴定中常用的一种方法[1,2].然而,当多个外源基因被导入植物基因组中时,应用普通PCR分开检测则显得时间长成本高;而多重PCR(MPCR)可以克服上述...
A multiplex polymerase chain reaction (mPCR) was developed and evaluated for detection of pox viral infections simultaneously using clinical samples from sheep and goats. Specific primers for three pox viruses of sheep and goats including sheeppox virus (SPPV), goatpox virus (GTPV) and orf virus...
In this study, we developed a novel one-step multiplex polymerase chain reaction (mPCR) for the rapid detection of 10 pathogenic genes (stx1, stx2, eae, bfpA, invE, aggR, esth, estp, elt, and astA) of DEC. Five categorized strains were used as positive controls for DEC harboring each...
DNA isolated from Salmonella was amplified by multiplex polymerase chain reaction (mPCR), and the presence of Salmonella was determined by reading fluorescent signals from hybridization between probes on DNA sensors and fluorescently labeled target DNA using the Bio-Plex system. The developed multiplex ...
Samples were tested using multiplex polymerase chain reaction, followed by reverse line blot hybridisation. Results: We recruited 221 FSWs, (median age: 25 years). During the previous 3 months, 30% reported never using condoms; only 2.7% always used condoms. Of 217 women with urine samples, ...
Abstract A multiplex polymerase chain reaction (PCR) was designed for the simultaneous detection of three viruses involved in reproductive and respiratory ... DXH Li - 《Molecular & Cellular Probes》 被引量: 28发表: 2013年 Establishment and application of a multiplex PCR assay for simultaneous dete...
PURPOSE: Despite the availability of molecular methods, identification of the causative virus in children with acute respiratory infections (ARIs) has proven difficult as the same viruses are often detected in asymptomatic children. METHODS: Multiplex reverse transcription polymerase chain reaction assays we...
A multiplex polymerase chain reaction (PCR) assay was developed for the identification of Salmonella enterica serovar Typhimurium. Three sets of primers we... YH Lim,K Hirose,H Izumiya,... - 《Japanese Journal of Infectious Diseases》 被引量: 221发表: 2003年 A multiplex real-time PCR for ...