一、引言 多重PCR( multiplex polymerase chain reaction,MPCR)也称复合PCR,是在常规PCR基础上改进并发展起来的一种新型PCR扩增技术,即可在一个反应体系中加入两对以上引物,同时扩增出多个核酸片段,由 Chambehian'于1988年首次提出,其反应原理、反应试剂和操作过程与常规PCR相同。多重PCR既有单个PCR的特异性和敏感性,...
MULTIPLEX POLYMERASE CHAIN REACTION, ArtículoOften, studies require the polymerase chain reaction (PCR) amplification of several nucleic acid targets. Examples include the amplification of several exons of the same gene for mutation detection (e.g., p53 or dystrophin) (1–4), the amplification of...
1. 多重聚合酶链锁反应 藉由多重聚合酶链锁反应(multiplex polymerase chain reaction)进行毒性因子(PVL基因)及SCCmec分型;利用微量琼脂稀释法(a… ir.cmu.edu.tw|基于3个网页 2. 多重聚合酶连锁反应 慧智临床基因医... ... 聚合酶连锁反应( Polymerase Chain Reaction)多重聚合酶连锁反应(Multiplex Polymerase...
1) multiplex polymerase chain reaction多引物聚合酶链2) multiplex PCR 多引物PCR 1. Methods Sixty eye bank donors and 54 recipients were selected randomly before operation,and herpes simplex virus type 1(HSV-I),type 2(HSV-II),cytomegalovirus(CMV) and varicella-zoster virus(VZV) were detected by...
The invention discloses a multiplex polymerase chain reaction (PCR) amplification method and a reagent kit for multiplex PCR amplification. The multiplex PCR amplification method comprises the step of designing public primers and multiplex primers with the public primers. The public primer design ...
Multiplex PCR (Polymerase Chain Reaction) method for detecting proximal SNP (Single Nucleotide Polymorphism)The invention discloses a multiplex PCR (Polymerase Chain Reaction) method for detecting proximal SNP (Single Nucleotide Polymorphism) loci and relates to a multiplex DNA amplification technology. ...
1) multiplex polymerase chain reaction多引物聚合酶链2) multiplex PCR 多引物PCR 1. Methods Sixty eye bank donors and 54 recipients were selected randomly before operation,and herpes simplex virus type 1(HSV-I),type 2(HSV-II),cytomegalovirus(CMV) and varicella-zoster virus(VZV) were detected by...
This study aimed to evaluate the occurrence of Streptococcus pneumoniae and Haemophilus influenzae in sputum of patients with community-acquired pneumonia (CAP) using culture and multiplex polymerase chain reaction (M-PCR) methods and to survey the antibiotic resistance patterns of aforesaid isolates. Res...
Multiplex polymerase chain reaction for the simultaneous detection and typing of polyomavirus JC, BK and SV40 DNA in clinical samples. J Virol Methods. 1999 Oct;82(2):137-44Fedele CG, Ciardi M, Delia S, et al. Multiplex polymerase chain reaction for the simultaneous detection and typing of ...
This work aims to develop a multiplex polymerase chain reaction combined with DNA pooling for mass screening for rare blood types.BackgroundThe differences in most blood group antigens are associated with single-nucleotide polymorphisms (SNPs), which are used in detecting blood antigen expression at ...