Oxidative Stress 8-isoprostane ELISA Kit 8iso1 96T
扩增产物经胶回收纯化后,按pEASY-T3 Zero Cloning Kit操作说明,把相应的片段连接到质粒载体中,再转化入Trans1-T1 phage Resistant感受态细胞,利用蓝白斑筛选获得阳性克隆菌株,挑取含有阳性克隆的单克隆菌落扩大培养,并送至生工生物工程(上海)股份有限公司测序鉴定。经鉴定为阳性质粒的,用NanoDrop 2000测定质粒浓度,利用双...
Additionally, no studies have investigated how age and AMH affect mtDNA copy number, and whether these effects are independent of each other. Therefore, we aim to scrutinize the effect of age on mtDNA copy number and AMH level, concomitantly exploring the possible effect of age and AMH on ...
The measurement of mtDNA copy number was done by droplet digital PCR. Quantitative determination of insulin, adiponectin, TNF-α and chemerin in serum/plasma was performed by flow-through fluorometry and commercial ELISA kit. Statistical analysis and graphs were obtained in R Statistical Software (...
The measurement of mtDNA copy number was done by droplet digital PCR. Quantitative determination of insulin, adiponectin, TNF-α and chemerin in serum/plasma was performed by flow-through fluorometry and commercial ELISA kit. Statistical analysis and graphs were obtained in R Statistical Software (...
All cells were tested for mycoplasma contamination using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich, MP0040A). Method details Cell line generation Generation of knock-out (KO) cell lines To generate MTFP1 CRISPR/Cas9-mediated gene KO, U2OS cells were transfected with ps-Cas9-2A-...
For RT-PCR, first-strand cDNA was synthesized by using the PrimeScriptTM RT rea- gent Kit with gDNA Eraser (TaKaRa), and PCR was run for 35 cycles (94 °C for 30 s, 58 °C for 30 s and 72 °C for 30 s) in a 25-μl volume containing 10 ng of template ...
Multiplex pools were sequenced using MiSeq Reagent Kit v3.0 (Illumina, CA, USA) in paired-end, 250 bp reads. Postrun data as FASTQs, limited to reads with QV ≥ 30, were exported for analysis. NGS of nanobiopsied mitochondria was compared to lysate obtained from single-cells ...
DNA was extracted from formaldehyde-fixed mouse tissue using the TIANamp FFPE DNA Kit (TIANGEN, DP331, Beijing, China) according to the manufacturer’s instructions. qPCR was conducted using SYBR Green (4309155, Life Technologies Corporation, Gaithersburg, MD, USA) and performed on ABI 7500 PCR ...
The PCR product was cloned using a GeneJet PCR cloning kit (Fermentas, Burlington, Ontario, Canada), and the recombinant plasmid was purified using a PureLink Quick Plasmid Miniprep kit (Invitrogen, Carlsbad, CA, USA). Clones were analyzed in-house on a capillary ABI3100 Genetic Analyzer (...