首先,作者使用2种互补的方法将APEX送至hTR邻近标记位点,第一是将hTR连接至噬菌体MS2 RNA茎环(stem loop),使其特异地且强有力地连上MCP-APEX2,第二是用无活性的Cas13-APEX2和对应的gRNA靶向到hTR,但是结合力较弱。 接着,作...
摘要:【目的】为了探讨lncRNA DANCR在肿瘤细胞中的作用机制,本研究拟构建一种新的MS2-RIP方法用于鉴定DANCR的相互作用分子。【方法】利用MS2噬菌体衣壳蛋白可与噬菌体复制酶编码基因5’端一段由19个碱基组成的RNA茎环结构序列,即MS2结合...
‘nucleotide stem’’ loop region(pae site)located at the 57 terminus of the MS2 bacteriophage replicase gene.The specific binding of phage MS2 capsid protein and pac site have been applied for detecting of RNA in living cells as calibrator,RNA delivery vector and as standard,calibrator and ...
· nome by capsi d protei n is initiated by the bi ndi ng of the capsid protein di mer to a specif i c 19 ’— nucl eoti de stem— — loop region( pac site) located at the 5 terminus of the MS2 bacter i ophage replicase gene.The specif i c binding of pha ge M S 2 ...
最后,7SK 6xMS2 stemloop地区与本地子,克隆到pVitro - MS2 - YFP的免入息审查贷款计划; pVitro - MS2 - BP - YFP - 7SK 6xMS2。 翻译结果2复制译文编辑译文朗读译文返回顶部 最后,7SK 6xMS2 stemloop 地区,与本机启动子,被 subcloned 到 pVitro-MS2-YFP-NLS ;pVitro-MS2-BP-YFP-7SK-6xMS2。
aNext, a 6× repeat of the MS2 binding stem loop was subcloned in between the 7SK promoter and coding region. 其次, MS2约束词根圈的6×重覆是在7SK促进者和编制程序区域之间subcloned。[translate]
Figure 1. Inserting MS2 stem-loop sequences into an endogenous locus (A) The MS2/MCP live imaging system visualizes transcription using RNA stem-loop repeats that are bound by the MS2-coat protein (MCP) fused to a fluorescent protein. (B) MS2 stem-loops can be inserted into different endo...
whichindicatesthattheGFP—MS2rtargetmRNA—MS2bindingstemloopsystemis arobustandeffectivewaytoimagemRNAinlivingcells. [Keywords] GFP—MS2;invivoimagingofmRNA;RNA—proteininteraction;Jun RNA在生物体内发挥着重要作用,它不仅能单独行使功能,还能和DNA、蛋白质形成复合物来执行多种生物学功能,如转录、转录后调控等...
求翻译:Next, a 6× repeat of the MS2 binding stem loop was subcloned in between the 7SK promoter and coding region.是什么意思?待解决 悬赏分:1 - 离问题结束还有 Next, a 6× repeat of the MS2 binding stem loop was subcloned in between the 7SK promoter and coding region....