Figure 1. Inserting MS2 stem-loop sequences into an endogenous locus (A) The MS2/MCP live imaging system visualizes transcription using RNA stem-loop repeats that are bound by the MS2-coat protein (MCP) fused to a fluorescent protein. (B) MS2 stem-loops can be inserted into different endo...
(Empty Backbone)3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences. plenti Backbone size (bp)10221 Vector type Mammalian Expression, Lentiviral, CRISPR PromoterU6 and EF1A Se...
aNext, a 6× repeat of the MS2 binding stem loop was subcloned in between the 7SK promoter and coding region. 其次, MS2约束词根圈的6×重覆是在7SK促进者和编制程序区域之间subcloned。[translate]
最后,7SK 6xMS2 stemloop地区与本地子,克隆到pVitro - MS2 - YFP的免入息审查贷款计划; pVitro - MS2 - BP - YFP - 7SK 6xMS2。 翻译结果2复制译文编辑译文朗读译文返回顶部 最后,7SK 6xMS2 stemloop 地区,与本机启动子,被 subcloned 到 pVitro-MS2-YFP-NLS ;pVitro-MS2-BP-YFP-7SK-6xMS2。
3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences. 产品编号 产品名称 规格 价格 ZY8061 lenti sgRNA(MS2)_zeo backbone 2ug 特别提示:本公司的所有产品仅可用于科研实验,严禁用...
质粒名称:lenti sgRNA(MS2)_zeo backbone 质粒编号:61427 质粒备注: (Empty Backbone)3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences. ...
of the MS2 replicase mRNA, which folds into a hairpin loop structure. This hairpin loop is recognized with high specificity and affinity by the MS2 bacteriophage capsid RNA-binding protein MS2 (Kd of 3-300 × 10 9 , depending on the stem loop sequence and the MS2 RBP ...
‘nucleotide stem’’ loop region(pae site)located at the 57 terminus of the MS2 bacteriophage replicase gene.The specific binding of phage MS2 capsid protein and pac site have been applied for detecting of RNA in living cells as calibrator,RNA delivery vector and as standard,calibrator and ...
It is well characterized that nucleic acids and other small molecules can be packaged in the MS2 VLP via their interaction with a short RNA stem-loop structure (19 nucleotides: pac site), which in turn binds to coat protein dimers5,31,32,33. Thus, the interaction with the 5’-terminus ...
(a) MS2 wt sequence from the bacteriophage. Nucleotide positions are relative to the translation start codon AUG (+1). (b) MBSORF loops are dimers of 2 different loop sequences distanced by 20 nts, repeated every 50 nts. MBSORF is C-variant (cytosine at position -5). The stem loop ...