Figure 1. Inserting MS2 stem-loop sequences into an endogenous locus (A) The MS2/MCP live imaging system visualizes transcription using RNA stem-loop repeats that are bound by the MS2-coat protein (MCP) fused to a fluorescent protein. (B) MS2 stem-loops can be inserted into different endo...
(Empty Backbone) 3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences. BACKBONE Vector backboneplenti Vector typeMammalian Expression, Lentiviral, CRISPR PromoterU6 and EF1A ...
Thus, to label a specific mRNA in vivo, a tandem repetition of a 19-nucleotide-long stem-loop (SL) sequence derived from the MS2 OAS sequence (MSL) is transcriptionally fused to the RNA under investigation. The RNA is detected by the co-expression of fluorescent protein-tagged MS2 CP (...
LOCUS sgRNA(MS2) cloning backbone 3008 bp ds-DNA circular SYN 12-AUG-2019 DEFINITION sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.. ACCESSION . VERSION . KEYWORDS sgRNA(MS2) cloning backbone SOURCE synthetic DNA constru...
质粒名称:lenti sgRNA(MS2)_puro backbone质粒编号:73795质粒备注: (Empty Backbone) 3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.载体名称:plenti载体大小:10221载体类型:哺乳...
sequence of bacteriophage MS2 RNA:primary and secondary 杆菌BL21(DE3)后用IPTG诱导表达,形成了内含反 structure of the replicase gene[J1.Nature,1976,260:500—507. 义RNA的MS2 VLP。源于HIV一1的Tat转导肽具有 【2】 van Meerten D,Olsthoom
3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences. 产品编号 产品名称 规格 价格 ZY8061 lenti sgRNA(MS2)_zeo backbone 2ug 特别提示:本公司的所有产品仅可用于科研实验,严禁用...
(a) MS2 wt sequence from the bacteriophage. Nucleotide positions are relative to the translation start codon AUG (+1). (b) MBSORF loops are dimers of 2 different loop sequences distanced by 20 nts, repeated every 50 nts. MBSORF is C-variant (cytosine at position -5). The stem loop ...
In order to achieve high-affinity binding to Qβ CP, the Qβ stem-loop must encompass a 3 nt loop with an 8 bp stem (Witherell and Uhlenbeck, 1989). The only nucleotide within this sequence whose identity is essential for this interaction, provided that the base pairing within the stem ...
最后,7SK 6xMS2 stemloop地区与本地子,克隆到pVitro - MS2 - YFP的免入息审查贷款计划; pVitro - MS2 - BP - YFP - 7SK 6xMS2。 翻译结果2复制译文编辑译文朗读译文返回顶部 最后,7SK 6xMS2 stemloop 地区,与本机启动子,被 subcloned 到 pVitro-MS2-YFP-NLS ;pVitro-MS2-BP-YFP-7SK-6xMS2。