Figure 1. Inserting MS2 stem-loop sequences into an endogenous locus (A) The MS2/MCP live imaging system visualizes transcription using RNA stem-loop repeats that are bound by the MS2-coat protein (MCP) fused to a fluorescent protein. (B) MS2 stem-loops can be inserted into different endo...
CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos 来自 dx.doi.org 喜欢 0 阅读量: 185 作者:C Hoppe,HL Ashe 摘要: CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe ...
(Empty Backbone)3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences. plenti Backbone size (bp)10221 Vector type Mammalian Expression, Lentiviral, CRISPR PromoterU6 and EF1A Se...
Structures of the Mutant CPs Complexed with the MS2 Stem-Loop The structures of all of the CPs in complex with MS2 stem-loops are essentially identical to those seen in wild-type phage capsids, with small differences in the conformations of flexible lysine and arginine side chains on the inne...
产品类别:质粒/载体 lenti sgRNA(MS2)_zeo backbone 载体 质粒类型: 慢病毒表达载体,CRISPR/Cas系统 启动子: EF1a 载体大小: 9996 bp (查看载体序列) 载体抗性: Ampicillin 筛选标记: Zeocin 备注: 3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resist...
Moreover, it shows an ordered genome that is shaped as a branched network of connected RNA stem-loops, of which the majority interacts with the inside of the capsid. The RNA–CP interactions are primarily located on one side of the capsid, which might have consequences for genome packaging ...
(a-c) Schematic representation of different MS2 Stem Loops. (a) MS2 wt sequence from the bacteriophage. Nucleotide positions are relative to the translation start codon AUG (+1). (b) MBSORF loops are dimers of 2 different loop sequences distanced by 20 nts, repeated every 50 nts. MBSORF...
lenti sgRNA(MS2)-puro backbone质粒载体 >> 质粒交货期: 现货索取资料及报价 交货期: 现货 产品介绍 质粒名称:lenti sgRNA(MS2)_puro backbone质粒编号:73795质粒备注: (Empty Backbone) 3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance ...
LOCUS sgRNA(MS2) cloning backbone 3008 bp ds-DNA circular SYN 12-AUG-2019 DEFINITION sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.. ACCESSION . VERSION . KEYWORDS sgRNA(MS2) cloning backbone SOURCE synthetic DNA constru...
The RNA forms a branched network of stem-loops that almost all allocate near the capsid inner surface, while predominantly binding to coat protein dimers that are located in one-half of the capsid. This suggests that genomic RNA is highly involved in genome packaging and virion assembly. MS2 ...