Moreover, it appears that these stem-loop structures are able to bind in a similar fashion to the coat protein dimer as the wild-type operator hairpin. The EM map also shows additional density between the potential operator-binding sites, linking the RNA stem-loops together to form an ...
The reconstruction shows the double-stranded helices in the stem loop structures (e). At some positions individual NA's connecting to the capsid are resolved, as shown by fitting of the X-ray structure of the 19-nucleotide TR (magenta) bound to the capsid (blue) (pdb:1ZDH) in the EM ...
a MS2-RIP method was designed and conducted to identify the molecules that interact with DANCR.【Methods】The specific binding of MS2 bacteriophage capsid protein and MS2 binding site (MS2 BS), a 19-base RNA stem-loo...
It is well characterized that nucleic acids and other small molecules can be packaged in the MS2 VLP via their interaction with a short RNA stem-loop structure (19 nucleotides:pacsite), which in turn binds to coat protein dimers5,31,32,33. Thus, the interaction with the 5’-terminus of ...
Figure 1. Inserting MS2 stem-loop sequences into an endogenous locus (A) The MS2/MCP live imaging system visualizes transcription using RNA stem-loop repeats that are bound by the MS2-coat protein (MCP) fused to a fluorescent protein. (B) MS2 stem-loops can be inserted into different endo...
The reconstruction shows the double-stranded helices in the stem loop structures (e). At some positions individual NA's connecting to the capsid are resolved, as shown by fitting of the X-ray structure of the 19-nucleotide TR (magenta) bound to the capsid (blue) (pdb:1ZDH) in the EM ...
stem’’ loop region(pae site)located at the 57 terminus of the MS2 bacteriophage replicase gene.The specific binding of phage MS2 capsid protein and pac site have been applied for detecting of RNA in living cells as calibrator,RNA delivery ...
high—afinityinteractionbetweenRNAbacteriophagecoatproteinMS2anditscognatebindingstemloop.Byconstructing pcDNA3.1一Jun—Ms2-48XvectorwhichcontainedtheJuncDNAand48repeatsofMs2bindingsites.theJunmRNAcould befluorescentlylabeledandvisualizedbytheinteractionofGFP—MS2fusionproteinwiththestemloopstructureoftheJun-- MS2-48...
aNext, a 6× repeat of the MS2 binding stem loop was subcloned in between the 7SK promoter and coding region. 其次, MS2约束词根圈的6×重覆是在7SK促进者和编制程序区域之间subcloned。[translate]
最后,7SK 6xMS2 stemloop地区与本地子,克隆到pVitro - MS2 - YFP的免入息审查贷款计划; pVitro - MS2 - BP - YFP - 7SK 6xMS2。 翻译结果2复制译文编辑译文朗读译文返回顶部 最后,7SK 6xMS2 stemloop 地区,与本机启动子,被 subcloned 到 pVitro-MS2-YFP-NLS ;pVitro-MS2-BP-YFP-7SK-6xMS2。