Order NO. B532453 miRNA 第一链 cDNA 合成(茎环法) 英文名miRNAFirst Strand cDNA Synthesis (Stem-loop Method) 相关类别反转录PCR(RT-PCR)储存冷冻(-20℃) 编 号包装库存目录价(￥)您的价格(￥)数量 B532453-002020 RXNS现货900900 B532453-005050 RXNS现货22002200 ...
The invention involves construction of a vector comprised of single stranded sequences that form "stem-loop" structures wherein the "loop" comprises the single stranded target sequence and the "stem" comprises the double stranded "functional" cis-acting genetic elements or recognition sequences. The...
miRNAFirst Strand cDNA Synthesis (Stem-loop Method)|MIRNA 第一链 CDNA 合成(茎环法) 基本信息更多信息 中文名称:MIRNA 第一链 CDNA 合成(茎环法) 中文同义词:MIRNA 第一链 CDNA 合成(茎环法) 英文名称:miRNAFirst Strand cDNA Synthesis (Stem-loop Method) ...
[33] force field while the non-bonded interactions, solvation, torsion angle preferences are derived from statistics. This energy function and the corresponding loop modeling method are adopted in the Modeller program. Rohl et al. [48] and later Mandell et al. [49] used the Rosetta scoring ...
Experimental method D: Stem-loop poly(U) tailed RT-PCR sRNA ID sRNA Sequence (5'-->3') Length (bp) GC (%) Let-7a UGAGGUAGUAGGUUGUAUAGUU 22 36.36 RTprimer(universal) GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAAAAAAAAAAAAAAAAVN 62 40.32 VN = V: A, C and G,N: A, C, G ...
1.Introduction Instem-loop poly(A) tailed RT-PCRmethod, total RNAs, including miRNAs, piRNA, or siRNA, etc. are extended by a poly(A) tailing reaction using poly(A) Polymerase and ATP. The sncRNA with a poly(A) tail is converted into cDNA through reverse transcription primed by aunivers...
This in-line cleavage method identifies nucleotides that are conformationally flexible enough to result in in-line attack of the 2′OH to cleave the phosphodiester bond [23]. In-line probing of S2MG35U resulted in a two-fold decrease in cleavage at positions U25, U30 and A33 along with ...
Development and application of a simple loop-mediated isothermal amplification method on rapid detection of Listeria monocytogenes strains. Mol. Biol. Rep. 2011, 39, 445–449. 5. Gandelman, O.A.; Church, V.L.; Moore, C.A.; Kiddle, G.; Carne, C.A.; Parmar, S.; Jalal, H.; Tisi,...
GraphPad software 9 was used to analyze the data. Student’s t test was used to analyze comparisons between two groups. One-way ANOVA was used to analyze comparisons between multiple groups. Survival curves were generated using the Kaplan‒Meier method and compared using the log-rank test. Al...
the 3′-UTR of COMMD1 mRNA was examined for microRNA target sites using the mirSVR scoring method.34,35 Three target sites corresponding to miR-205, miR-491-5p, and miR-7, respec- tively, were predicted (Supplementary Figure S1a); of these, the miR-205 target site exhibited good mirSV...