SORT LNP是模块化的,可以通过工程化配方的开发搭建成熟的SORT LNP平台。 2023年,得克萨斯大学西南医学中心在Nature Protocols发表文章:《Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods...
SORT LNP是模块化的,可以通过工程化配方的开发搭建成熟的SORT LNP平台。 2023年,得克萨斯大学西南医学中心在Nature Protocols发表文章:《Preparation of selective organ-targeting (SORT)lipid nanoparticles (LNPs) using multipletechnical methods for tissue-specific mRNAdelivery》,报道了详细的SORT LNP制备步骤,包...
org/10.1093/nar/gks588. 9.Burke, P.A., Gindy, M.E., Mathre, D.J., Kumar, V., Prud’homme, R.K., 2013. Preparation of Lipid Nanoparticles. US 2013/0037977. 10. Buschmann, M.D., Carrasco, M.J., Alishetty, S., Paige, M., Alameh, M.G., Weissman, D., 2021. Nanomateria...
org/10.1093/nar/gks588. 9.Burke, P.A., Gindy, M.E., Mathre, D.J., Kumar, V., Prud’homme, R.K., 2013. Preparation of Lipid Nanoparticles. US 2013/0037977. 10. Buschmann, M.D., Carrasco, M.J., Alishetty, S., Paige, M., Alameh, M.G., Weissman, D., 2021. Nanomateria...
参照Moderna和BioNtech的新冠疫苗配方,以ALC-0315、MC3、SM102为主要脂质,制备包载mRNA的脂质纳米颗粒(LNP)。 实验原理: ALC-0315、MC3、和SM102是三种可用于人体的可解离脂质,在酸性条件下可质子化形成阳离子脂质,通过静电作用和带负电的mRNA结合,形成载有mRNA的脂质纳米颗粒(lipid nanoparticles, LNPs)。本实验中采...
9.Burke, P.A., Gindy, M.E., Mathre, D.J., Kumar, V., Prud’homme, R.K., 2013. Preparation of Lipid Nanoparticles. US 2013/0037977. 10. Buschmann, M.D., Carrasco, M.J., Alishetty, S., Paige, M., Alameh, M.G., Weissman, D., ...
RNAPreparation.IntJMolSci.2022Oct31;23(21):13247.doi: 10.3390/ijms232113247. [64]LeeKH,KimS,SongJ,etal.EfficientcircularRNAengineeringby end-to-endself-targetingandsplicingreactionusingTetrahymenagroupIintron ribozyme.MolTherNucleicAcids.2023Aug1;33:587-598.doi: 10.1016/j.omtn.2023.07.034. [65]...
9.Burke, P.A., Gindy, M.E., Mathre, D.J., Kumar, V., Prud’homme, R.K., 2013. Preparation of Lipid Nanoparticles. US 2013/0037977. 10. Buschmann, M.D., Carrasco, M.J., Alishetty, S., Paige, M., Alameh, M.G., Weissman, D., ...
9.Burke, P.A., Gindy, M.E., Mathre, D.J., Kumar, V., Prud’homme, R.K., 2013. Preparation of Lipid Nanoparticles. US 2013/0037977. 10. Buschmann, M.D., Carrasco, M.J., Alishetty, S., Paige, M., Alameh, M.G., Weissman, D., ...
微流控技术将过程控制技术(Process Control Technology,PCT)与过程分析技术(Process Analytical Technology,PAT)相结合,可实现良好的在线样品制备技术(On-line Preparation Technology,OPT)。样品在连续化制备的过程中,工艺过程中参数完全可控,且具备良好重现性,所以较传统分步割裂式制备、分批次生产的方法来说更具有可放大...