mRNA represents a promising new vaccine technology platform with high flexibility in regard to development and production. Here, we demonstrate that vaccines based on sequence optimized, chemically unmodified mRNA formulated in optimized lipid nanoparticles (LNPs) are highly immunogenic and well tolerated ...
Unmodified mRNA in LNPs constitutes a competitive technology for prophylactic vaccines. NPJ vaccines 2017; 2(1): 29.Lutz J, Lazzaro S, Habbeddine M, Schmidt KE, Baumhof P, Mui BL, et al. Unmodified mRNA in LNPs constitutes a competitive technology for prophylactic vaccines. NPJ Vaccines (...
Pyroptosis, a lytic form of cell death mediated by the gasdermin family, is characterized by cell swelling and membrane rupture. Inducing pyroptosis in cancer cells can enhance antitumor immune responses and is a promising strategy for cancer therapy. Ho
ANEXT安龄生物·巨噬细胞外泌体协助mRNA-LNPs疗法治疗难治性胰腺疾病 ANEXT安龄生物·外泌体每周速递037 ANEXT安龄生物·外泌体每周速递036 ANEXT安龄生物·工程化细胞外囊泡助力缓解急性肺损伤小鼠肺部炎症 ANEXT…
该策略主要在β细胞中诱导蛋白质表达,β细胞是位于胰岛中的胰岛素生成细胞。此外,腹膜巨噬细胞细胞外囊泡(EVs)转移促进了mRNA的递送,并且功效与全身毒性无关。总之,这些数据数据表明mRNA-LNPs是一种可行的非病毒方法,可在难以转染的胰腺细胞中诱导蛋白质表达。
Finally, we tested hOTC-mRNA/LNPs. After intravenous injection of naked hOTC mRNA only, Fluc-mRNA/LNP, or hOTC-mRNA/LNP into mice, we performed western blotting for hepatic hOTC expression. hOTC-mRNA/LNP treatment induced hepatic hOTC protein expression, whereas there were no bands in the live...
Lipid nanoparticle (LNP)-formulated mRNA vaccines were rapidly developed and deployed in response to the SARS-CoV-2 pandemic. Due to the labile nature of mRNA, identifying impurities that could affect product stability and efficacy is crucial to the long
Harnessing the power of the immune system to target cancer cells is one of the most appealing approaches for cancer therapy. Among these immunotherapies, messenger ribonucleic acid (mRNA) cancer vaccines are worthy of consideration, as they have demonstr
Harnessing the power of the immune system to target cancer cells is one of the most appealing approaches for cancer therapy. Among these immunotherapies, messenger ribonucleic acid (mRNA) cancer vaccines are worthy of consideration, as they have demonstr
LNPs are produced by mixing biomolecules and lipids, which self-assemble to form the desired structure. In this domain, microfluidics shows clear advantages: high mixing quality, low-stress conditions, and fast preparation. Studies of LNPs produced in micromixers have revealed, in certain ranges of...