2.ORF密码子优化:平衡翻译效率与稳定性 密码子优化需兼顾宿主tRNA丰度(通过密码子适应指数CAI)与mRNA结构稳定性(通过最小自由能MFE或平均未配对概率AUP)。关键方法包括: 线性设计(LinearDesign):结合动态规划与格解析算法,快速生成高稳定性ORF序列,实验显示其设计的新冠疫苗序列抗体效价提升128倍。 RiboTree模型:基于蒙...
「miRNA建库方式」有别于其它组学,主要是利用其长度分布在20 nt左右,文库构建时「切胶回收」该范围的条带,条带中还包含rRNA,siRNA,piRNA,tRNA等非编码RNA,所有后续分析会使用Rfame数据库,miRBASE来剔除非miRNA。 「miRNA生信分析重点关注」case组和control组之间差异表达miRNA,差异表达miRNA的靶mRNA预测,也会功能分析...
假尿苷-5'-三磷酸(Pseudo-UTP)用于赋予所需的mRNA特征,例如增加的核酸酶稳定性,增加的翻译或先天免疫受体与体外转录的RNA的相互作用的改变。假UTP与5-甲基胞苷-5'-三磷酸(5-甲基-CTP)一起显示出在培养和体内的先天免疫抑制,同时在近的出版物中增强了翻译。 Karikó发现tRNA结构中有假尿苷(Pseudo-UTP)不会引发...
图4 HSV病毒基因组 此外，当病毒驻留神经元，处于潜伏状态时，基因组会变成环状，转录非常少的RNA，包括2个LATRNA，8个miRNA。miRNA在抑制早期病毒蛋白ICP0，ICP4，ICP34.5表达方面起着非常重要的作用。图5 潜伏期HSV病毒基因转录 核衣壳（Capsid）包裹dsDNA的二十面体衣壳(icosapentahedral capsid)，由162个壳粒...
poly(A)-tail, the most unique and pivotal feature of IVT mRNA setting the term “modified” (mod-mRNA) relies on replacement of natural prime nucleosides (A, T, G, C, U) with modified base derivatives that are partly presented in multiple types of RNA (mRNA, rRNA, tRNA and viral RNA...
Protein structure preference, tRNA copy number, and mRNA stem/loop content. Luo Liaofu,Jia Mengwen,Li Xiaoqin. Biopolymers . 2004Protein structure preference, tRNA copy number, and mRNA stem/loopcontent. Luo, L,Jia, M,Li, X. Biopolymers . 2004...
[25,26,118]. In addition to describing the Nsp1-40S interactions, these structural studies also found 43S pre-initiation complexes (PICs), some including the eIF2-tRNAi-GTP ternary complex, bound to Nsp1, demonstrating that Nsp1 permits complete 43S formation. The way that Nsp1 interacts ...
.19Mature mRNA includes the coding region, UTR, the poly(A) tails, and the 5′ cap that can be recognized by ribosomes and carried by tRNA to create proteins. As in DNA, genetic information in mRNA is contained in the sequence of nucleotides that are arranged into codons consisting of ...
tRNA ligase structure reveals kinetic competition between non-conventional mRNA splicing and mRNA decay. Elife 8, 213 (2019). Article Google Scholar Naudin, C. et al. PUMILIO/FOXP1 signaling drives expansion of hematopoietic stem/progenitor and leukemia cells. Blood–2016–10–747436–35 (2017)...
By optimizing the mRNA sequence (changes in codons, matching the frequency of host transfer RNA, (tRNA) or as a determinant for the introduction of secondary structures), the rate of protein synthesis and half-life of mRNA function can be altered. In improving mRNA translation, 1mΨ nucleotide...