2006. Structural basis for mRNA and tRNA positioning on the ribosome. Proc. Natl. Acad. Sci. U. S. A. 103:15830 -15834.Structural basis for mRNA and tRNA positioning on the ribosome - Berk, Zhang, et al. - 2006Berk, V., W. Zhang, R. D. Pai, and J. H. Cate. 2006. ...
detected by the Q-PCR method.gInfluence of rifampin on the mRNA stability of six represented genes in the WT or KO Msm strains.h,iThe KEGG annotation analysis revealed that 49 genes in the metabolic pathway were significantly differentially expressed between WT RNase J and its knockout strain....
Importance of an N-terminal structural switch in the distinction between small RNA-bound and free ARGONAUTE ARGONAUTE (AGO) is the core protein component of small RNA-guided silencing complexes. Free, but not RNA-bound, AGO turns over rapidly. The authors identify a structural AGO switch whose...
Instead, when a misincorporation occurs, the mismatched tRNA–mRNA pair moves to the peptidyl (P) site and, from this location, causes a reduction in the fidelity at the A site, triggering post-peptidyl transfer quality control. This reduced fidelity allows for additional incorrect tRNAs to be...
The cryo-EM structural differences between tmRNA complexes with and without S1 suggested that RpsA was potentially involved in unwinding the single-stranded part of the open-reading-frame (ORF) in mRNA-like domain (MLD) [7]. Immunoblot assay and UV-induced crossing-linking experiments showed that...
mRNA acts as a barrier to tRNA accommodation and translation elongation. The interaction between an m6A-modified codon and cognate tRNA echoes the interaction between a near-cognate codon and tRNA, because delay in tRNA accommodation depends on the position and context of m6A within codons and on...
to the differences in kcat values for cognate and near-cognate reactions, whereas the intrinsic affinity differences are not used for tRNA discrimination. Th... I Wohlgemuth,C Pohl,MV Rodnina - 《Embo Journal》 被引量: 61发表: 2014年 加载更多来源...
In state I, RNase R is bound between the head and the body of the 30S subunit, adjacent to the exit site of the mRNA channel (Fig.1a). With the exception of the C-terminal K/R-rich tail, the density for RNase R was sufficient to unambiguously assign all domains of the protein (Fi...
Here, using single-molecule, bulk kinetics and structural methods, we show that 2′-O-methylation within coding regions of mRNA disrupts key steps in codon reading during cognate tRNA selection. Our results suggest that 2′-O-methylation sterically perturbs interactions of ribosomal-monitoring bases...
(Supplementary Fig.8and9). The molecular basis for this isoacceptor-specificity appears to lie in the local topological differences between mtRNASer(GCU)and mtRNASer(UGA), with the 5’ portion of the canonical ‘T-loopSer(UGA)’ notably less arched and closer to the peptide backbone than ...