mRNA组就是测某个条件下上图中的mature mRNA,从上图可看出mRNA有一个Poly-A tail,mRNA测序文库构建时,利用「oligo(dT)磁珠能特异结合Poly-A tail」的原理,特异地将mRNA从总RNA中分离出来实现富集。「mRNA生信分析重点关注」case组和control组之间差异表达基因,也会做基因与蛋白互作、新转录本预测、可变剪切、基因...
Messenger RNA (mRNA) is a pivotal molecule of life, involved in almost all aspects of cell biology. As the subject of basic and applied research for more than 5 decades, mRNA has only recently come into the focus as a potentially powerful drug class able to deliver genetic information. Synt...
This paper reviews the current status of mRNA-based therapeutics used for various diseases in both preclinical and clinical stages. It also provides a reasonable discussion on key barriers in this field and an outlook towards its future.信使RNA(messenger RNA, mRNA)是一类携带遗传信息并能够直接指导...
A. The protein factors MBNL1 and U2AF65 bind alternative RNA structures to regulate splicing. Proc. Natl Acad. Sci. USA 106, 9203–9208 (2009). Article CAS PubMed PubMed Central Google Scholar Oberstrass, F. C. et al. Structure of PTB bound to RNA: specific binding and implications ...
SLC7A11 as a therapeutic target to attenuate phthalates-driven testosterone level decline in mice DEHP induces testosterone synthesis disorder in the testis.MEHP impairs mitochondrial structure and function in Leydig cells.MEHP disrupts glutathione meta... Y Zhao,XQ Wang,RQ Liu,... - 《Journal of...
- 《International Journal of Biochemistry & Cell Biology》 被引量: 0发表: 2015年 The structure ofEnterococcus faecalisthymidylate synthase provides clues about folate bacterial metabolism Drug resistance to therapeutic antibiotics poses a challenge to the identification of novel targets and drugs for the...
L1 mRNA expression is consistent in MCF7 single cell clusters.A. The number of million mapped reads per cell is shown for each tSNE cluster of MCF7 cells (ANOVA, ***,< 0.0001).B. The L1 expression level per cell quantified by RPM is shown for Clusters 1, 3, and 4 in the Violin...
Decapping of the mRNA is depicted by a lightning bolt in the cap structure; mRNA degradation ensues. Experimental Procedures Plasmids Reporter plasmids used in this study all contain the human β-globin gene, modified as schematized in Figure 3A (sequences available upon request), and inserted ...
An examination of mechanisms by which synonymous mutations may alter protein levels, structure and functions. in Single Nucleotide Polymorphisms: Human Variation and a Coming Revolution in Biology and Medicine (eds. Sauna, Z. E. & Kimchi-Sarfaty, C.) 99–132 (Springer International Publishing, ...
Nature Structural & Molecular Biology volume 25, pages 208–216 (2018)Cite this article 6392 Accesses 92 Citations 12 Altmetric Metrics details Abstract Chemical modifications of mRNA may regulate many aspects of mRNA processing and protein synthesis. Recently, 2′-O-methylation of nucleotides was ...