构建的模板文库,进行体外转录和修饰(3' polyA-tailing 和 5' m7G-capping),然后将 mRNA文库转染到细胞中,并通过barcode测序定量分析(图 1a),从而能够通过多核糖体分析直接测量翻译效率以及细胞或溶液中mRNA的降解。 UTRs序列设计 mRNA文库总共包含233条不同的mRNA序列。其中112...
The role of the open reading frame and its sequence composition has recently emerged as equally important in the control of gene expression as that of the 5′ and 3′UTRs, which have traditionally been seen as the regulatory hubs of the mRNA. More specifically, codon usage has been highlight...
The 5' stem loop binds P-TEFb as well as the Hexim1 protein, which acts to inhibit the kinase activity, while the 3' stem-loop binds the Larp7/PIP7S protein, which, in addition to a methylphosphate capping enzyme (Mepce), stabilizes the RNA [95–99,151,152]. For a long time the...