Therapeutic mRNAs and vaccines are being developed for a broad range of human diseases, including COVID-19. However, their optimization is hindered by mRNA instability and inefficient protein expression. Here, we describe design principles that overcome these barriers. We develop an RNA sequencing-bas...
In various species, multiple mRNA features have been reported to affect RNA decay. For instance, inE. coli, shorter mRNAs tend to exhibit greater stability13. Conversely, in Zebrafish, lengthening the 3’ UTR can suppress codon-mediated deadenylation, resulting in enhanced mRNA stability14. Apart ...
1D 1H NMR exhibits high sensitivity on probing structure change and protein-excipient interactions in solution and demonstrates strong potential as a relatively efficient method for formulation screening and assessment [30]. For example, NMR and multivariate analysis have been combined to study quaternary...
Its half-life is reduced when those cells are exposed to unsaturated fatty acids, indicating that the 5′-exoribonuclease encoded by the XRN1 gene is required for the rapid degradation of the OLE1 transcript but is not required for fatty acid-induced destabilization....
Gerstel B, Tuite MF, McCarthy JE (1992) The effects of 5’ capping, 3’polyadenylation and leader composition upon the transational stability of mRNA in a cell-free extract derived from the yeast Saccharomyces cerevisiae. Mol Microbiol 6: 2339–2348...
(MK2−/−) mice showing attenuated production of tumor necrosis factor (TNFα) protein when compared to wild-type mice. The mRNA levels, however, in wild-type mice were quite similar as compared to MK2−/−mice, indicating regulation at the translational level which might be imparted...
Internal m6A residues that follow G in mRNA can be labelled and quantified in a 2D TLC method61. The relative abundance of m6A versus (A + C + U) in 400 ng mRNA that was either left untreated (−FTO) or incubated for 1 h with 1 μM bacterially expressed recombinant...
different LCLs, indicating the reliability of the RNA half-life measurements. (3) The array- measured interindividual RNA half-life differences for a random set of genes were validated by qPCR using independent cell cultures, suggesting that systematic bias is unlikely. Because trans-acting fac- ...
The primed γδ T17 cells were used for m6A-RNA immunoprecipitation sequencing (RIP-seq) analysis, and 11,705 m6A peaks were detected in 6,021 mRNAs of these cells (Table S1C). The distribution of m6A methylation sites was located primarily in the 5′ terminal capping area, coding ...
(C). Using a linear RNA in situ hybridization method to detect osk transcripts, it becomes evident that there is a drastic decrease in oskar mRNA at the posterior in the cup alleles. cup16/Df(2L)bsc7 and cup20/Df(2L)bsc7 have no apparent localization of oskar, while there is a ...