Differentially displayed cDNAs are isolated, cloned, sequenced and used as probes on northern blots to confirm differences in the particular mRNA level. The general strategy seems to be simple and straightforward, although increasing number of reports on methodical refinements (Liang et al. 1993; Li...
SARS-CoV-2 is a single-stranded, non-segmented, positive-sense RNA with both 5´cap and a tail at 3´end [32], [33]. The SARS-CoV-2 RNA is similar to cellular mRNA, which is an essential evolutionary advantage, by mimicking the cellular mRNA, directly driving viral RNA for transl...