The cap-to-tail guide to mRNA turnover.doi:10.1038/35067025The levels of cellular messenger RNA transcripts can be regulated by controlling the rate at which the mRNA decays. Because decay rates affect the expression of specific genes, they provide a cell with flexibility in effecting rapid ...
An evolutionarily conserved mRNA-degradation pathway is initiated by the removal of the 3′ poly(A) tail. This disrupts the translation initiation complex and provides degradative enzymes with access to the 5′ cap and remaining RNA body.
methods to efficiently identify and quantify RNA isoforms genome-wide in single cells are still lacking. Here, we introduce single cell RNA Cap And Tail sequencing (scRCAT-seq), a method to demarcate the boundaries of isoforms based on short-read sequencing, with higher efficiency and lower cost...
The 5’CapQ Assay is a novel, leading technology that enables measurement of capped and tailed intact mRNA and 5′ capping efficiency.
10 Physical and Functional Interactions between the mRNA Cap Structure and the Poly(A) Tail The 5′-end of all nuclear-encoded messenger RNAs in eukaryotes contains a cap structure (m 7 GpppX). With few exceptions, the 3′-end also contains a poly(A) tail. These structures are added by...
[8] Lai WC, Zhu M, Belinite M, Ermolenko DN, et al. Intrinsically Unstructured Sequences in the mRNA 3' UTR Reduce the Ability of Poly(A) Tail to Enhance Translation. J Mol Biol. 2022 Dec 30;434(24):167877. doi: 10.1016/j.jmb.2022.167877. ...
[8] Lai WC, Zhu M, Belinite M, Ermolenko DN, et al. Intrinsically Unstructured Sequences in the mRNA 3' UTR Reduce the Ability of Poly(A) Tail to Enhance Translation. J Mol Biol. 2022 Dec 30;434(24):167877. doi: 10.1016/j.jmb.2022.167877. ...
这个吴小波说得不是太专业, mRNA 的5‘cap是主要是为了让核糖体结合上去,有了5’cap和3‘polyA tail的RNA才是mRNA,mRNA疫苗里面降低天然免疫的是假尿嘧啶。
Co-injection of poly(A)-binding protein (PABP) mRNA increased the function of the cap and poly(A) tail in promoting translation from poly(A)(+) but not poly(A)(-) mRNAs, particularly for mRNAs containing secondary structure. In the absence of an internal ribosome entry site, expression ...
[C]便帽; 帽子 a soft flat covering for the head that has a curved part sticking out at the front as a peak and is worn especially as part of a uniform [C]保护盖〔套〕 a protective covering for the end or top of an objectv.(动词) vt. 覆盖,笼罩 put a cap on sth to cover the...