Mouse Tnf qPCR Primer Pair,即小鼠Tnf qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),是一种...
Quantitative RT-PCR (qPCR) analysis was performed using Taqman MicroRNA Assays (ABI, Carlsbad, CA, USA) and SYBR RT- PCR kits (Takara Shuzo, Otsu, Japan). The relative expression level of miR-124 was normalized to that of internal control sno234. The primers for TNF-α were: 5′-AAG...
Quantitative RT-PCR (qPCR) analysis was performed using Taqman MicroRNA Assays (ABI, Carlsbad, CA, USA) and SYBR RT- PCR kits (Takara Shuzo, Otsu, Japan). The relative expression level of miR-124 was normalized to that of internal control sno234. The primers for TNF-α were: 5′-AAG...
interferon alpha (IFNα), were enriched substantially later in infection (14 dpi in Fig.2h). Infected mice at 14 dpi also showed activated pDCs (Fig.2iand Supplementary Fig.2e) marked by CCR7 and CD83 expression44. This coincided with chronically sustained interferon response and sustained infla...
The qPCR primers sequences of the primers used are Mouse Il1β F: 5′-GCA ACT GTT CCT GAA CTC AAC T-3′, R: 5′-ATC TTT TGG GGT CCG TCA ACT-3′. Mouse Il6 F: 5′-GCT TAA TTA CAC ATG TTC TCT GGG AAA-3′, R: 5′-CAA GTG CAT CAT CGT TGT TCA TAC-3′. Mouse Tnf...
Primers are listed in Supplementary Table 3. Histopathology Organs were formalin-fixed, dehydrated in 70% EtOH, paraffin-embedded and H&E stained. Whole blood CBCs were performed on a Scil Vet abc Animal Blood Counter (Scil Animal Care). Statistics Quantified results of qPCR and flow ...
Applied Biosystems). The expression levels of TMEM119, CX3CR1, P2RY12, OCT-4, hIL-1β, hTNF-α, hIL-6, hARG1, hMCP-1 mRNAs were normalized to that of RPL13A and mIl-1β, mTnf-α, mIl-6, mArg1, mMcp-1 were normalized to that of mGapdh. Primer sequences are listed in ...
Standard molecular assays and histopathology scoring were performed as previously described [51,52,53]. Details of these procedures and a list of qPCR primers are found in the Supplemental experimental procedures. Statistical analyses Non-sequencing-based statistical analyses were performed with Prism 10...
n = 5 mice/group. One-way ANOVA followed by Tukey’s post hoc test was used in panel A. Student’s t test was used in panel B. Additional file 10: Table S1. Sequences of primers used for qPCR. Additional file 11: File 1. Uncropped Western bot gel images....
Quantitative polymerase chain reaction reactions were performed with SYBR Advantage qPCR Premix (Clontech, Mountain View, CA). Expression levels were determined in triplicate per sample and normalized to the expression of glyceraldehyde 3-phosphate dehydrogenase. Primers used include: glyceraldehyde 3-...