Table S1. Primers for Bead Synthesis, Related to Figure 1 Table S2. Oligonucleotides Used in Library Construction, Related to Figure 1 Table S3. Tissue Digestion Methods and Analyzed Cell Numbers, Related to Figure 2 Table S4. Markers and Annotation for 88 Main Clusters Defined by MCA Data,...
g qPCR for the relative expression of CDH1, ACTA2, Collagen I, Collagen II, Fibronectin and TGFB1 mRNA in A549 cells, with or without TGF-β1 treatment for 48 h. h Immunofluorescence staining for E-cadherin and Collagen I expression in A549 cells, with or without 10 ng/mL TGF-β...
The TF specificity in adult tissues was further validated by qPCR (Supplementary Fig. 2e and Supplementary Data 2). Importantly, TF specificity at the mRNA levels is largely consistent with their DNA-binding activities. Transcription network of the NRs and other TF families. Studies on the mRNA ...
THX mice mount neutralizing antibody responses toSalmonellaor severe acute respiratory syndrome coronavirus 2 Spike S1 receptor-binding domain, with blood incretion of human cytokines, including APRIL, BAFF, TGF-β, IL-4 and IFN-γ, all at physiological levels. These mice can also develop lupus ...
The specific primers are shown in Additional file 1: Table S3, and qPCR was performed according to the procedure of qPCR method as above with a different annealing temperature at 61 ℃. The GAPDH (GenBank BC096440.1) gene from mouse was selected as the housekeeping gene, and the relative ...
(n = 16 slices for each group).ETranscriptional levels ofSirt1,Sirt3,Tgfb1, andSmad3genes in the ovaries were measured by qRT-PCR (n = 3 replicates from 3 mice).FDiagram of the isolation of ovarian stromal cells.G,HqRT-PCR was conducted to validate the purity of the ...
Primers were used at 0.4 μM final concentration, and their sequences are reported in Table S5. qPCR amplification program: 1 cycle, 15 min 95°C; 50 cycles, 15 s 94°C–30 s 60°C–30 s 72°C; 1 cycle for melting curve. Depending on the expression level of the target genes ...
All qPCR results are expressed as relative ratios of the target cDNA to Gapdh transcripts normalized to that ratio in the reference condition, which always corresponds to heterozygote Pax3GFP/+embryos. Primers used for detecting specific transcripts were designed with Primer3 (see Additional file 4...
Fetal growth restriction (FGR) affects 5–10% of pregnancies, and can have serious consequences for both mother and child. Prevention and treatment are limited because FGR pathogenesis is poorly understood. Genetic studies implicate KIR and HLA genes in
Nkrp1g þ gd T cells also expressed transcripts for TGF-b1 and TGF-b3, as well as for the keratinocyte growth factor (Supplementary Figure S9d). Differential expression of Nkg2a could be confirmed by flow cytometry, with Nkg2a preferentially expressed on Nkrp1g À gd IELs (Figure 6c)...