DNA PrimersMembrane GlycoproteinsCD40 LigandCloning, MolecularSequence AlignmentBase SequenceSequence Homology, Amino AcidAccording to studies in human and mouse species it is apparent that both CD40 ligand and tumor necrosis factor alpha (TNF alpha) are members of a novel family of cytokines, referred...
The systems-wide quantification of the N-glycoproteome with integrated mass spectrometry platforms has revealed distinct levels of regulation of IR in mouse adipocytes. We designed the strategy to differentiate between changes in total protein levels, relative N-glycan occupancy, or changes in N-glycan...
Acknowledgements We thank Gabriel Fernández for the transgenic mouse colony management, and Micaela Sureda and Irith Bergsma for their initial contribution to this work. The authors gratefully acknowledge the Advanced Bioimaging Unit at the Institut Pasteur de Montevideo for their support and assistance ...
A high stromal collagen density pro- duces a threefold increase in mouse mammary tumor numbers with a more invasive phenotype, producing greater local invasion and metastasis [10]. In humans, mammographic studies have identified, in normal breasts, areas with a low adipocyte content and a high ...
2.2. Primer Design We performed multiple sequence alignment of all isoforms’ nucleotide sequences for both TNFRSF1A and TNFRSF1B genes using MUltiple Sequence Comparison by Log-Expectation (MUSCLE) in UniPro UGENE. As TNFRSF1A and TNFRSF1B genes are not well annotated, we selected primers ...
upregulated TSLP expression is considered a hallmark of AD pathogenesis [14,15]. TNF-α is a pro-inflammatory cytokine that promotes the production of multiple inflammatory cytokines and chemokines [23]. TNF-α was highly expressed in a mouse model of DNCB-induced contact allergy [24], and it...