Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine® (EK0527) View all TNF antibodies and products Macrophage Activation Related Products Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0767) Septic Shock Related Products ...
There are many instances in which the efficacy of a therapeutic protein is limited by an unwanted immune reaction to the therapeutic protein. Several mouse monoclonal antibodies have shown promise as therapeutic agents in a number of human disease settings, but in certain cases have failed due to ...
DNA PrimersMembrane GlycoproteinsCD40 LigandCloning, MolecularSequence AlignmentBase SequenceSequence Homology, Amino AcidAccording to studies in human and mouse species it is apparent that both CD40 ligand and tumor necrosis factor alpha (TNF alpha) are members of a novel family of cytokines, referred...
The systems-wide quantification of the N-glycoproteome with integrated mass spectrometry platforms has revealed distinct levels of regulation of IR in mouse adipocytes. We designed the strategy to differentiate between changes in total protein levels, relative N-glycan occupancy, or changes in N-glycan...
Acknowledgements We thank Gabriel Fernández for the transgenic mouse colony management, and Micaela Sureda and Irith Bergsma for their initial contribution to this work. The authors gratefully acknowledge the Advanced Bioimaging Unit at the Institut Pasteur de Montevideo for their support and assistance ...
primer on the 5′ side of the deletion was: GGT GGA GAG CCT GCC GGC CG and the primer on the 3′ side of the deletion was: GGC AGC CGA TGG CGT GGA ATC TG, and cDNA was generated using an oligo-dT primer from total RNA from different tissues. The cDNAs were separated by agar ...
the transfected cell media inhibited up to 70% of the binding of TNFα. The recombinant TNF receptor derivative was next tested for inhibition of TNFα biological activity. A sensitive bioassay for TNFα is a measurement of cytolysis of mouse WEHI 164 (clone 13) cells. The transfected cell ...
primer on the 5′ side of the deletion was: GGT GGA GAG CCT GCC GGC CG and the primer on the 3′ side of the deletion was: GGC AGC CGA TGG CGT GGA ATC TG, and cDNA was generated using an oligo-dT primer from total RNA from different tissues. The cDNAs were separated by agar ...
A high stromal collagen density pro- duces a threefold increase in mouse mammary tumor numbers with a more invasive phenotype, producing greater local invasion and metastasis [10]. In humans, mammographic studies have identified, in normal breasts, areas with a low adipocyte content and a high ...
Scheme 1. Primers’ binding sites and amplicons. Table 1. TNFRSF1A isoforms’ primer table. Table 2. TNFRSF1B isoforms’ primer table. Table 3. Housekeeping genes’ primer table. 2.3. Cell Line Culturing with TNFα We used MCF-7, an epithelial-like cell line derived from invasive...