This is a terminal procedure unlike aqueous taps in humans which are minimally invasive and can be repeated many times. For aqueous taps, pulled glass micropipettes require a slightly narrower tip with an inner diameter of 80 µm and a corresponding outer diameter of 100 µm (...
Orthologs with high expression in both humans and mice included the key neutrophil genesCSF3R(encoding the G-CSF receptor),CXCR2,NCF4(neutrophil cytosolic factor 4), the transcription factorsMCL1,SPI1(encoding PU.1, an essential transcription factor for terminal granulopoiesis34,35) andJUNB, a tr...
Blood, organ and tissue collection Terminal organs, brain regions, and blood samples from subject and control C57BL/6 mice were collected after 24 hours, or 6 weeks (42 days) post 10-day social stress, and 24 hours or 1.5 weeks post 5-day social stress. Brains of C57BL/6 mice were ...
Seipel K, Medley QG, Kedersha NL, Zhang XA, O'Brien SP, Serra-Pages C, Hemler ME, Streuli M: Trio amino-terminal guanine nucleotide exchange factor domain expression promotes actin cytoskeleton reorganization, cell migration and anchorage-independent cell growth. Journal of cell science. 1999, ...
We conclude that diverse neuron types share a coordinated transcriptional program involving genes that facilitate maintenance, elaboration, or subcellular transport to the axon and presynaptic terminal. Neuronal types characterized by extensive axonal arbors, long-distance axonal projections, and/or faster fi...
The introduction of organic solution into in vitro cell cultures is a terminal protocol; thus, new samples are needed for each time point of the experiment. PP is most commonly selected for such applications due to its relative ease of use and efficiency; however, it necessitates new cell ...
Next, we asked whether the direct cardiac effects of DPP-4 inhibitors may be influenced by the metabolic milieu. Accordingly, we studied the hearts of aged, diabetic, high fat diet-fed (DM-HFD) mice. At the end of the study period, as expected, both body weight and blood glucose [9]...
Blood collection from the saphenous vein. are good methods of terminal blood collection when an aseptic sample is collecting blood from the tail in mice vein compared to blood obtained from needed. over 28 days old and from the orbital other sites7. sinus in mice of all ages. Anesthesia is...
[8]. The terminal differentiation of the theca externa is characterized by expression of contractile proteins that play critical roles leading to follicle rupture in the ovulation process [9]. On the other hand, terminal differentiation of the theca interna is characterized by development of theca ...
Terminal in- verted repeat sequences located at each end of a transpo- son are bound by the transposase, which mobilizes the element and mediates its insertion at a new locus. Several systems have been used in mice, each with their own effi- ciencies and insertion biases...