158 Phenotypic screening of a library of compounds using cell death as a readout identified ON1231320 as a PLK2-specific small molecule inhibitor. The identified isoform-selective PLK2 inhibitor ON1231320 caused apoptotic cell death in cultured tumor cell lines and showed significant tumor growth ...
Human non-small lung cancer cell (NSCLC) line H2087 (purchased in American Type Culture Collection, ATCC) was used as a model. Cells were grown at 37 °C in 5% CO2humidified atmosphere to 80% confluency in RPMI 1640 medium with L-glutamine (Mediatech) supplemented with 10% foetal bovine ...
Mouse models of non|[ndash]|small cell lung cancer (NSCLC) based on serial orthotopic transplantation of tumor tissueMouse studies suggest serial passages of tumor tissues in nude mice could help model NSCLC.SciBX: Science-Business eXchange
Preclinical models of non-small cell lung cancer (NSCLC) require better clinical relevance to study disease mechanisms and innovative therapeutics. We sought to compare and refine bioluminescent orthotopic mouse models of human localized NSCLC. Athymicnudemice underwent subcutaneous injection (group 1-SC,...
Activity of panitumumab against mutant and wild type EGFr NSCLC cell lines and xenografts 4494 Background. Epidermal growth factor receptor (EGFr) is a transmembrane tyrosine kinase expressed on many different tumor types. Recent evidence suggests that Non-Small Cell Lung Carcinoma (NSCLC) patients wi...
In addition to modeling chromosomal rearrangements in cell lines and ES cells, viral delivery of the CRISPR-Cas9 system to generate the Eml4-Alk rearrangement in non-small-cell lung cancers (NSCLCs) [22, 39] has been reported in mouse models. Our groups have recently shown that hydrodynamic ...
Use of bioluminescent imaging to investigate the role of nuclear factor-κΒ in experimental non-small cell lung cancer metastasis Nuclear factor (NF)-κB is frequently over-expressed in non-small cell lung cancer (NSCLC), but the exact role of this observation remains unclear. In this... GT...
EML4-ALK is a fusion-type protein tyrosine kinase that is generated in human non-small-cell lung cancer (NSCLC) as a result of a recurrent chromosome inversion, inv (2)(p21p23). Although mouse 3T3 fibroblasts expressing human EML4-ALK form transformed foci in culture and s.c. tumors in ...
Indeed, studies of mouse MC38 tumor models as well as human colorectal cancer, NSCLC, and breast cancer demonstrate EZH2 expression and associated H3K27me3 marking of tumor-infiltrating Tregs. This in turn suggests that targeting EZH2 expression in intratumoral Tregs might be a potentially ...
Vimentin is highly expressed in metastatic cancers, and its expression correlates with poor patient prognoses. However, no causal in vivo studies linking vimentin and non–small cell lung cancer (NSCLC) progression existed until now. We use three complem