embryoids suggests that iCdx2ESCs exhibit restricted ExE differentiation potential. In the natural embryo, the ExE can be subdivided into proximal ExE (adjacent to the EPI/ExE boundary) and distal ExE (toward the tip of the embryo). The proximal ExE is characterized by the expression of TSC-...
emerging spatial transcriptome approaches such as Geo-seq4, seqFISH5, DBiT-seq6, Stereo-seq7, and sci-Space8, have disentangled the molecular architecture of embryo development by mapping the spatial organization
6 Differentiation overview and scRNAseq analysis metrics. a) Mouse differentiation overview, with comparison across protocols. Quality control and pre/post filtering numbers for each mouse timepoint from the various protocols in table. b–d) Micrographs of cells during the embryoid body (EB), ...
Recently, scRNA-seq and other single-cell genomic approaches have been used to investigate how the molecular landscape of cells within the mouse embryo changes during early development. These methods have provided insights into how symmetry breaking of the epiblast population leads to commitment to dif...
23 (PB011): scRNA-seq and single-cell spatial transcriptome of diffuse-type gastric cancer mouse model reveals a novel interaction between neutrophils and cancer cellsdoi:10.1016/j.ejca.2024.114551M. KakiuchiH. KokuboH. InoueA. Tsubosaka
and ABE-treated embryos showed higher number of off-target structure variations, while CBE-treated embryos had similar number of SVs compared with that of the control group (Fig.1a and Additional file 1: Fig. S1b), likely spontaneous structure variations during embryo development. ...
Code and data used for the publication. Contribute to IStevant/XY-mouse-gonad-scRNA-seq development by creating an account on GitHub.
The expression of Thy1 in the epidermis, along with the expression of a CD3/Vγ3/Vδ1 TCR complex, has been characterized as a defining feature of dendritic epidermal T cells (DETCs)31,32,33,34,35. In the developing mouse embryo, Vγ3/Vδ1 TCR/Thy1+T cells arise in the fetal ...
Figure S7. Comparison of differential expression changes with a published bulk RNA-seq study. Figure S8. Overview of the Tet-TKO chimaera assay. Figure S9. Mapping, cell type assignments and embryo staging for Tet-TKO scRNA-seq samples. Figure S10. Differential gene expression analysis between ...
Analysis of ChIP-seq targets Pre-calculated z-scores for expression throughout preimplantation embryo development were utilised from Boroviak et al., scRNA-seq data84. A custom script with R (v4.2.1) base functions85 and ggplot2 v3.4.086 were used for data exploration and visualisation. Expre...