β-actin mouse primer 序列 β-actin(β-肌动蛋白)是一种高度保守的紧张蛋白,在多种细胞类型中广泛表达,具有重要的细胞骨架结构和功能。由于其表达水平稳定且在多种实验研究中被广泛应用,β-actin的引物序列和扩增方法成为了常用的内参控制方法之一。以下是β-actin鼠引物的序列: 前向引物:5'-AGCCATGTACGTAGCC...
Beta-actin(β-actin)是一种细胞骨架蛋白质,在细胞的结构和功能中起着重要作用。β-actin在多个细胞类型中广泛表达,并且其表达水平相对稳定。因此,β-actin常用作实时定量PCR(qPCR)或RT-qPCR的内参基因,来校正不同样本之间的变异。 要使用β-actin小鼠引物序列进行PCR实验,首先需要设计适当的引物。设计引物时,需要考...
β-actin mouse primer序列-回复 在分子生物学和基因表达研究中,基因的测定和定量是非常重要的。为了确定一个基因的表达水平,研究人员通常使用RT-PCR(逆转录聚合酶链反应)技术来扩增目标基因的片段,并对扩增的产物进行测定和定量分析。在RT-PCR中,引物是扩增过程中的关键因素之一。β丝氨酸(β-actin)是一种常用的...
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PCR primer sequences are indicated in Table 1 and were designed using Primer Express software (Applied Biosystems, Foster City, CA). Amplification reactions were performed in 25 μl reaction volume containing 1 μl cDNA, 12.5 μl of 2 × SYBR green master mix (Qiagen, Valencia, CA), 9.5 ...
Each value was normalized to b-actin. The relative expression level of the genes was calculated using the DDCt method. The sequences of PCR primers were as follows: E2f1: F5′-CTGCAGCAACTGCAGGAGAG-3′; R5′-CTCCGAAAGCAGTTGCAGC-3′; E2F2: F5′-ACGGCGCAACCTACAAAGAG-3′; R5′-GTC...
Primer sequences are available on request. Metabolic phenotyping experiments Temperature was monitored daily using a rectal probe (Physitemp)62. Briefly, the probe was lubricated with glycerol and was inserted 1.27 cm (1/2 inch) and temperature was measured when stabilized. Glucose monitoring, ...
产品名称:Mouse ?-Actin RT-PCR Primer .0订购此产品 供应商:Agilent 规格: 目录价:￥:2957.92 库存状态: CAS编号: 应用范围: 种属来源: 相关信息: 保存条件: 说明书地址:点击查看详细 打印此页关闭此页 上一个:Human G6PD RT-PCR Primer[302047 /] ...
fused and expressed at the C-terminus in the AAV-SpRY-ABE8e-C-terminal plasmid. The Pde6b-rd10 c.1678C>T targeted gRNA was installed into the BsmBI sites of the AAV-SpRY-ABE8e-C-terminal plasmid via the Golden Gate method, with the protospacer sequence embedded in the primers. The ...
NRG-1 was quantitated by densitometry and values were normalized to total β-actin (Right panel). Data were means ± SEM, n = 3. ***P < 0.001 vs. pLVX-Ctl, ###P < 0.001 vs. si-Con. c miR-193b-5p binding sites in the circNRG-1 sequence were predicted. d ...