Beta-actin(β-actin)是一种细胞骨架蛋白质,在细胞的结构和功能中起着重要作用。β-actin在多个细胞类型中广泛表达,并且其表达水平相对稳定。因此,β-actin常用作实时定量PCR(qPCR)或RT-qPCR的内参基因,来校正不同样本之间的变异。 要使用β-actin小鼠引物序列进行PCR实验,首先需要设计适当的引物。设计引物时,需要考...
β-actin mouse primer 序列β-actin mouse primer 序列 β-actin(β-肌动蛋白)是一种高度保守的紧张蛋白,在多种细胞类型中广泛表达,具有重要的细胞骨架结构和功能。由于其表达水平稳定且在多种实验研究中被广泛应用,β-actin的引物序列和扩增方法成为了常用的内参控制方法之一。以下是β-actin鼠引物的序列: 前向...
上海玉博生物科技有限公司 主要致力于“Mouse/Rat beta-Actin Primer Pair R&D-RDP-105-025”的生产销售。多年的“Mouse/Rat beta-Actin Primer Pair R&D-RDP-105-025”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大用户信赖。欢
To compare the effects of CLN3 protein isoforms on autophagic flux we used a tandem acid-stable red and acid-labile green fluorescent protein (RFP, GFP) sensor fused to the autophagosome-associated protein, microtubule-associated protein light chain 3 beta (GFP-RFP-LC3B) in HEK-293 cells. ...
appropriate primers through ABI prism 7000 Sequence Detector (Applied Biosystem) with Thunderbird SYBR Green qPCR Mix (Toyobo). Relative mRNA expression was calculated using the ΔΔCt method and each gene was normalized with Ct value of β-actin. All primers are listed in Supplementary Table3....
β-actin mouse primer序列-回复 在分子生物学和基因表达研究中,基因的测定和定量是非常重要的。为了确定一个基因的表达水平,研究人员通常使用RT-PCR(逆转录聚合酶链反应)技术来扩增目标基因的片段,并对扩增的产物进行测定和定量分析。在RT-PCR中,引物是扩增过程中的关键因素之一。β丝氨酸(β-actin)是一种常用的...
(Applied Biosystems) with SYBR Select Master Mix (Applied Biosystems, 4,472,908). qPCR data were analyzed by the ΔΔCT method by normalizing the expression of each gene to housekeeping gene β-Actin and then to the control groups. Primer sequences for mouse are listed in Additional file1:...
Deposition of amyloid beta (Aβ) and hyperphosphorylated tau along with glial cell-mediated neuroinflammation are prominent pathogenic hallmarks of Alzheimer’s disease (AD). In recent years, impairment of autophagy has been identified as anoth
et al. FAD mutant PS-1 gene-targeted mice: increased A beta 42 and A beta deposition without APP overproduction. Neurobiol. Aging 23, 335–348 (2002). Article CAS PubMed Google Scholar Izuo, N. et al. An App knock-in mouse inducing the formation of a toxic conformer of Abeta as ...
Nishimoto, N.et al. Heterocomplex formation by Arp4 and beta-actin is involved in the integrity of the Brg1 chromatin remodeling complex.J Cell Sci125, 3870–3882 (2012). ArticleCASPubMedGoogle Scholar Lee, K.et al. Expansion of chromosome territories with chromatin decompaction in BAF53-dep...