β-actin mouse primer 序列 β-actin(β-肌动蛋白)是一种高度保守的紧张蛋白,在多种细胞类型中广泛表达,具有重要的细胞骨架结构和功能。由于其表达水平稳定且在多种实验研究中被广泛应用,β-actin的引物序列和扩增方法成为了常用的内参控制方法之一。以下是β-actin鼠引物的序列: 前向引物:5'-AGCCATGTACGTAGCC...
Beta-actin(β-actin)是一种细胞骨架蛋白质,在细胞的结构和功能中起着重要作用。β-actin在多个细胞类型中广泛表达,并且其表达水平相对稳定。因此,β-actin常用作实时定量PCR(qPCR)或RT-qPCR的内参基因,来校正不同样本之间的变异。 要使用β-actin小鼠引物序列进行PCR实验,首先需要设计适当的引物。设计引物时,需要考...
β-actin mouse primer序列-回复 在分子生物学和基因表达研究中,基因的测定和定量是非常重要的。为了确定一个基因的表达水平,研究人员通常使用RT-PCR(逆转录聚合酶链反应)技术来扩增目标基因的片段,并对扩增的产物进行测定和定量分析。在RT-PCR中,引物是扩增过程中的关键因素之一。β丝氨酸(β-actin)是一种常用的...
上海玉博生物科技有限公司 主要致力于“Mouse/Rat beta-Actin Primer Pair R&D-RDP-105-025”的生产销售。多年的“Mouse/Rat beta-Actin Primer Pair R&D-RDP-105-025”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大用户信赖。欢
RDP-105-025 Mouse/Rat beta-Actin Primer Pair (25 TESTS)RDP-106-025 Mouse/Rat GAPDH Primer Pair (25 TESTS)RDP-148-025 Human Nestin Primer Pair (25 TESTS)RDP-153-025 Mouse/Rat Nestin Primer Pair (25 TESTS)RDP-23-025 Human CCL5/RANTES Primer Pair (25 TESTS)RDP-319-025 Human DPPA5/...
To compare the effects of CLN3 protein isoforms on autophagic flux we used a tandem acid-stable red and acid-labile green fluorescent protein (RFP, GFP) sensor fused to the autophagosome-associated protein, microtubule-associated protein light chain 3 beta (GFP-RFP-LC3B) in HEK-293 cells. ...
appropriate primers through ABI prism 7000 Sequence Detector (Applied Biosystem) with Thunderbird SYBR Green qPCR Mix (Toyobo). Relative mRNA expression was calculated using the ΔΔCt method and each gene was normalized with Ct value of β-actin. All primers are listed in Supplementary Table3....
(mRNA), including Foxa1, Gata3, Krt14, Krt18, Stat3, and Stat5a, were validated by quantitative reverse transcription real-time polymerase chain reaction (qRT-PCR) using custom designed exon-spanning primers and SYBR Green reporter, normalising data to the expression values of beta-actin (Actb...
Mouse Acta2 qPCR Primer Pair,即小鼠Acta2 qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),是...
Primers used for amplifying Tmem106a were: 5'- ATGGGTAAGGCAGTCTCC-3' (sense) and 5'-TGCTGGTGACCTGGCCTA-3' (antisense); β-actin primers were 5'-GTG GGG CGC CCC AGG CAC CA-3' (sense) and 5'-CTT CCT TAA TGT CAC GCA CGA T TTC-3' (antisense). Western blotting Total protein ...