β-actin mouse primer 序列 β-actin(β-肌动蛋白)是一种高度保守的紧张蛋白,在多种细胞类型中广泛表达,具有重要的细胞骨架结构和功能。由于其表达水平稳定且在多种实验研究中被广泛应用,β-actin的引物序列和扩增方法成为了常用的内参控制方法之一。以下是β-actin鼠引物的序列: 前向引物:5'-AGCCATGTACGTAGCC...
Beta-actin(β-actin)是一种细胞骨架蛋白质,在细胞的结构和功能中起着重要作用。β-actin在多个细胞类型中广泛表达,并且其表达水平相对稳定。因此,β-actin常用作实时定量PCR(qPCR)或RT-qPCR的内参基因,来校正不同样本之间的变异。 要使用β-actin小鼠引物序列进行PCR实验,首先需要设计适当的引物。设计引物时,需要考...
上海玉博生物科技有限公司 主要致力于“Mouse/Rat beta-Actin Primer Pair R&D-RDP-105-025”的生产销售。多年的“Mouse/Rat beta-Actin Primer Pair R&D-RDP-105-025”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大用户信赖。欢
RDP-344-025 Mouse/Rat Sla Primer Pair (25 TESTS)RDP-345-025 Mouse/Rat GATA-4 Primer Pair (25 TESTS)RDP-38-025 Human beta-Actin Primer Pair (25 TESTS)RDP-39-025 Human GAPDH Primer Pair (25 TESTS)RDP-58-025 Human/Mouse Fas Ligand/TNFSF6 Primer Pair (25 TESTS)RDPC1 Oligo (dT) ...
To compare the effects of CLN3 protein isoforms on autophagic flux we used a tandem acid-stable red and acid-labile green fluorescent protein (RFP, GFP) sensor fused to the autophagosome-associated protein, microtubule-associated protein light chain 3 beta (GFP-RFP-LC3B) in HEK-293 cells. ...
β-actin mouse primer序列-回复 在分子生物学和基因表达研究中,基因的测定和定量是非常重要的。为了确定一个基因的表达水平,研究人员通常使用RT-PCR(逆转录聚合酶链反应)技术来扩增目标基因的片段,并对扩增的产物进行测定和定量分析。在RT-PCR中,引物是扩增过程中的关键因素之一。β丝氨酸(β-actin)是一种常用的...
appropriate primers through ABI prism 7000 Sequence Detector (Applied Biosystem) with Thunderbird SYBR Green qPCR Mix (Toyobo). Relative mRNA expression was calculated using the ΔΔCt method and each gene was normalized with Ct value of β-actin. All primers are listed in Supplementary Table3....
(Applied Biosystems) with SYBR Select Master Mix (Applied Biosystems, 4,472,908). qPCR data were analyzed by the ΔΔCT method by normalizing the expression of each gene to housekeeping gene β-Actin and then to the control groups. Primer sequences for mouse are listed in Additional file1:...
et al. FAD mutant PS-1 gene-targeted mice: increased A beta 42 and A beta deposition without APP overproduction. Neurobiol. Aging 23, 335–348 (2002). Article CAS PubMed Google Scholar Izuo, N. et al. An App knock-in mouse inducing the formation of a toxic conformer of Abeta as ...
Primers for real-time PCR in this study are as shown in Supplementary information, Table S2. ELISA To determine the secretion of human MMP1, cell culture supernatants were collected after 48 h of culture. The levels of human MMP1 protein were measured by a Human MMP1 ELISA Kit (Cusabio...